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Oe33 cells

Manufactured by Merck Group
Sourced in Sao Tome and Principe

The OE33 cells are a human esophageal adenocarcinoma cell line. They are derived from the Barrett's esophagus-associated adenocarcinoma of the lower esophagus. The OE33 cells can be used for in vitro studies related to esophageal cancer research.

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2 protocols using oe33 cells

1

Cell Line Authentication and Characterization

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EAC cell lines OE19, OACM5.1C, ESO26, KYAE-1, and FLO-1 were purchased from the European Collection of cell cultures and authenticated by SNP arrays (2012). OE33 cells were purchased from Sigma (St. Louis, MO) and MKN7 cells from the Broad Institute and characterized within the Cancer Cell Line Encyclopedia project. Only low-passage cell aliquots of original stocks were used for experiments.
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2

Characterization of Gastroesophageal Cancer Cell Lines

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OE19 cells were obtained from the European Collection of Cell Cultures (ECACC), and OE33 cells were purchased from the Sigma (St. Louis, MO). OE19 is ERBB2-amplified gastroesophageal cancer cell line and sensitive to lapatinib, hence OE33 is ERBB2- and MET-amplified gastroesophageal cancer cell line and has intrinsic resistance to lapatinib. OE19 and OE33 were cultured in a humidified, 5% CO2 atmosphere at 37°C in Roswell Park Memorial Institute (RPMI-1640; GIBCO BRL, Grand Island, NY) medium supplemented with 10% fetal bovine serum. Tyrosine kinase inhibitors (lapatinib, saracatinib, and crizotinib) were purchased from the LC laboratories and were dissolved in dimethylsulfoxide (DMSO). Trastuzumab was purchased from the Department of Pharmacy at the Dana-Farber Cancer Institute.
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