Tumor tissues and organs, including the heart, liver, kidneys, lungs, and stomach, were harvested from the tumor-bearing mice, fixed in formalin, embedded in paraffin, and prepared as 2-mm thick sections. The sections were mounted on slides and histopathologically-stained with H&E. Tumor tissue sections were stained for infiltration by human T cells using a mouse monoclonal antibody against human CD3ε (Thermo Scientific, Waltham, MA, USA) and for tumor angiogenesis using a mouse monoclonal antibody against human CD31 (Abcam). Following overnight incubation with the primary antibody at 4°C, the secondary antibody was added, and the results were visualized using a ChemMate Envision Detection Kit (DakoCytomation, Carpinteria, CA, USA).
Mouse monoclonal antibody against human cd31
Manufactured by Abcam
Sourced in United States
Mouse monoclonal antibody against human CD31. This antibody recognizes the CD31 antigen, also known as PECAM-1, which is a transmembrane glycoprotein expressed on the surface of platelets, monocytes, neutrophils, and endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse monoclonal antibody against human cd31
1
Histopathological Tumor Tissue Analysis
2
Histopathological Analysis of Tumor Tissues
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Tumor tissues and organs, including the heart, liver, kidneys, lungs, and stomach, were harvested from the indicated tumor-bearing mice, xed with formalin, embedded in para n and prepared as 2-mm-thick sections. The sections were mounted on slides and histopathologically stained with HE. The tumor tissue sections were stained for in ltration by human T cells using a mouse monoclonal antibody against human CD3ε (Thermo Scienti c) and for tumor angiogenesis using a mouse monoclonal antibody against human CD31 (Abcam). Following overnight incubation with the primary antibody at 4°C, the secondary antibody was added, and the results were visualized using a ChemMate Envision Detection Kit (DakoCytomation).
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