high-performance liquid chromatography (HPLC) grade powder; CAS no.
104632-25-9), RT (95%; CAS no. 83-79-4), triethylamine (99%; CAS no.121-44-8),
no. 9003-39-8), bovine serum albumin (BSA; CAS no. 9048-46.8), potassium
phosphate monobasic (KH2PO4; CAS no. 7778-77-0),
sodium phosphate dibasic (Na2HPO4; CAS no. 7558-79-4),
perchloric acid (70%; CAS no.7601-90-3), MDA tetrabutyl ammonium salt
(96.08%; CAS no.100689-54-3), Bradford reagent (catalog no. B6916),
sulfosalicyclic acid (CAS no. 304851-84-1), and copper(II) nitrate
(Cu(NO3)2) (CAS number: 10031-43-3) were purchased
from Sigma-Aldrich, USA. 5,5′-Dithiobis(2-nitrobenzoic acid)
(DTNB; CAS no. 69-78-3), 1-chloro-2,4-dinitrobenzene (CDNB; CAS no.
74-11-3), GSH reduced (CAS no. 72-18-4), ethylenediaminetetraacetic
acid (EDTA; CAS no. 60-00-4), guanidine hydrochloride (CAS no. 50-01-1),
acetylthiocholine iodide (CAS no. 1866-15-5), 2,4-dinitrophenylhydrazine
(CAS no. 119-26-6), and sodium hydroxide (NaOH; CAS no. 7558-80-7)
were purchased from HIMEDIA Laboratories Private Limited in India.
Thiobarbituric acid (TBA; CAS no. 504-17-16) was purchased from Spectrochem,
Mumbai, India. All chemicals were of reagent grade and used as received.
Millipore water was used in the preparation of all of the aqueous
solutions.