Anti cd8a clone 53 6

Manufactured by BD

Anti-CD8a (clone 53–6.7) is a mouse monoclonal antibody that recognizes the CD8a antigen. CD8a is a cell surface glycoprotein that is expressed on a subset of T lymphocytes, known as cytotoxic T cells. The antibody can be used to identify and isolate CD8+ T cells in various immunological applications.

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Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions) Anti mouse tcr vβ screening panel, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Liberase, by Roche (1 mentions) Dnase 1, by Roche (1 mentions) Lysis buffer, by BioLegend (1 mentions) Anti cd45.2 clone 104, by BioLegend (1 mentions) Anti cd3 clone 17a2, by BioLegend (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Anti cd44 clone im7, by BD (1 mentions)

Close spelling variants of this product

Anti-CD8a (20 citations) CD8a (53-6.7, (18 citations) CD8a (clone 53-6.7, (17 citations) Clone 53–6.7 (10 citations) PE-conjugated anti-mouse CD8a (clone 53-6.7; (3 citations) Anti-mouse CD8a (clone 53-6.7, (3 citations) FITC-conjugated anti-CD8a (clone 53–6.7), (2 citations)

3 protocols using anti cd8a clone 53 6

Isolation and Analysis of Lymphocytes

Cited 3 times

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Lymphocytes were isolated from organs as described previously (13 (link)). Peripheral blood samples and
splenocytes were analyzed immediately or mixed with 10% DMSO and cryopreserved
at –80°C. Fluorochrome-labeled tetrameric complexes of
SSPPMFRV/H-2K^b (M38, residues 316–323) and
RALEYKNL/H-2K^b (IE3, residues 416–423) were generated in
house (17 (link)). Directly conjugated mAbs were
purchased from commercial vendors (BD Biosciences or BioLegend). Staining
procedures were described previously (29 (link)). OT-I cells were identified using anti-CD45.1 (clone A20),
anti-CD45.2 (clone 104), and anti-TCR Vα2 (clone B20.1). Differentiation
and proliferation were assessed using anti-CD8a (clone 53–6.7),
anti-CD127 (clone A7R34), anti-KLRG1 (clone 2F1), and anti-Ki67 (clone B56).
Repertoires were characterized at the protein level using an Anti-Mouse TCR
Vβ Screening Panel (BD Biosciences). Data were acquired using an LSR II
flow cytometer (BD Biosciences) and analyzed with FlowJo software versions 9.3
and 10.2 (Tree Star Inc.).

Smith C.J., Venturi V., Quigley M.F., Turula H., Gostick E., Ladell K., Hill B.J., Himelfarb D., Quinn K.M., Greenaway H.Y., Dang T.H., Seder R.A., Douek D.C., Hill A.B., Davenport M.P., Price D.A, & Snyder C.M. (2019). Stochastic Expansions Maintain the Clonal Stability of CD8+ T Cell Populations Undergoing Memory Inflation Driven by Murine Cytomegalovirus. The Journal of Immunology Author Choice, 204(1), 112-121.

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Murine Splenocyte Isolation and Flow Cytometry

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Splenocyte suspensions were prepared by passing the spleens directly through a 70 μM filter or by first dissecting them into small pieces and digesting them in RPMI containing 1 mg/ml Liberase (Roche) and 0.2 mg/ml DNase I (Roche) for 30 min at 37°C, prior to filtration. Red blood cells were removed using lysis buffer (Biolegend), and then cells were washed by centrifugation in PBS containing 1% foetal calf serum.
Single‐cell suspensions were incubated for 30 min at 4°C with Fc Block (anti CD16/CD32 clone 93, Biolegend) and H‐2Db tetramer (N396‐404) from the NIH Core Tetramer Facility. Cells were next stained with cell surface markers for 30 min at 4°C. The mAbs used were anti‐CD45.1 (clone A20, Tonbo Biosciences), anti‐CD45.2 (clone 104, Biolegend), anti‐CD8a (clone 53‐6.7, BD Pharmingen), anti‐CD3 clone (17A2, Biolegend), anti‐CD44 (clone IM7, BD Horizon) and anti‐PD1 (clone J43, BD Pharmingen). Samples were acquired on an LSRFortessa^TM (BD Biosciences). The data were analysed using FlowJo software.

Caddy S.L., Vaysburd M., Papa G., Wing M., O’Connell K., Stoycheva D., Foss S., Terje Andersen J., Oxenius A, & James L.C. (2020). Viral nucleoprotein antibodies activate TRIM21 and induce T cell immunity. The EMBO Journal, 40(5), e106228.

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Immunophenotyping of T cells and DCs

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Cells were incubated with FcBlock (supernatant of 2.4G2 cells with 5% of human serum) for 20 min. T cells were stained with anti-CD8a (clone 53,67) and anti-CD44 (clone IM7) (BD Biosciences®) in dark for 30 min. BMDCs were stained with anti-CD11c (clone HC3), anti-CD86 (clone Rit-GL1) and anti-MHCII (clone 2G9) (BD Biosciences®). Samples were acquired on flow cytometer FACS CANTO II (BD Biosci-ence®). Data were analyzed using FlowJo software (TreeStar).

do Nascimento de Freitas D., Gassen R.B., Fazolo T, & Souza A.P.D. (2016). Rapamycin increases RSV RNA levels and survival of RSV-infected dendritic cell depending on T cell contact. Toxicology in vitro : an international journal published in association with BIBRA, 36.

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