Gene expression analysis was carried out using Powerup™ SYBR Green PCR master mixes (Applied Biosystems). Expression of the gene of interest was normalised to TBP expression, using the 2-ΔΔCT method. Primer sequences are listed in Supplementary Information.
Powerup sybr green pcr master mixes
Manufactured by Thermo Fisher Scientific
Powerup™ SYBR Green PCR master mixes are ready-to-use solutions that contain all the necessary components for real-time PCR amplification and detection using SYBR Green fluorescence.
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Lab products found in correlation
2 protocols using powerup sybr green pcr master mixes
1
Gene Expression Analysis via qPCR
2
Gene Expression Analysis via qPCR
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Gene expression analysis was carried out using Powerup™ SYBR Green PCR master mixes (Applied Biosystems). Expression of the gene of interest was normalised to TBP expression, using the 2-ΔΔCT method. Primer sequences are listed in Supplementary Information.
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