Inserts were fixed in 1:10 buffered formalin for 48 h, and then for 24 h with fresh formalin before removal from BSL4 for processing and paraffin embedding. Sections stained by hematoxylin, and eosin (H&E) were imaged with an Evos XL core. Cells and sections stained for CD1a, CD11c, and virus antigen were imaged with an Olympus IS71 inverted fluorescence microscope. CD1a, CD11c, and EBOV glycoprotein were detected using respectively a mouse anti-CD1a (abcam 238,463; dilution: 1/100), -CD11c (abcam 52,632; dilution: 1/100), or a rabbit anti-EBOV GP pAb (IBT0301–015; dilution: 1/200) coupled to either a goat anti-rabbit AF594 or anti-mouse AF488 (Invitrogen; dilution: 1/200) as secondary antibody. DAPI staining (Sigma) was added to localize round cell nuclei and visualize the whole structure.
Is71 inverted fluorescence microscope
Manufactured by Olympus
The IS71 inverted fluorescence microscope is a high-performance instrument designed for a variety of applications in life science research. It features a fluorescence imaging system that allows for the visualization and analysis of fluorescently labeled samples, such as cells, tissues, and biological specimens. The IS71 is equipped with a range of optical components and illumination sources to provide clear, high-contrast images for researchers.
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Lab products found in correlation
2 protocols using is71 inverted fluorescence microscope
1
Immunohistochemical Analysis of Ebola Virus
2
Immunohistochemical Analysis of Ebola Virus
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Inserts were fixed in 1:10 buffered formalin for 48 h, and then for 24 h with fresh formalin before removal from BSL4 for processing and paraffin embedding. Sections stained by hematoxylin, and eosin (H&E) were imaged with an Evos XL core. Cells and sections stained for CD1a, CD11c, and virus antigen were imaged with an Olympus IS71 inverted fluorescence microscope. CD1a, CD11c, and EBOV glycoprotein were detected using respectively a mouse anti-CD1a (abcam 238,463; dilution: 1/100), -CD11c (abcam 52,632; dilution: 1/100), or a rabbit anti-EBOV GP pAb (IBT0301–015; dilution: 1/200) coupled to either a goat anti-rabbit AF594 or anti-mouse AF488 (Invitrogen; dilution: 1/200) as secondary antibody. DAPI staining (Sigma) was added to localize round cell nuclei and visualize the whole structure.
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