Log In Sign up
The largest database of trusted experimental protocols

Anti cd93 pe

Manufactured by BioLegend
Visit Supplier

Anti-CD93-PE is a fluorochrome-conjugated antibody that specifically binds to the CD93 cell surface antigen. CD93 is a transmembrane glycoprotein expressed on endothelial cells, monocytes, granulocytes, and platelets. This product can be used for the detection and analysis of CD93-expressing cells by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur, by BD (2 mentions) Pna biotin, by Vector Laboratories (2 mentions) Anti b220 percp, by BD (2 mentions) Pe anti cd138, by BD (2 mentions) Anti cd21 apc, by BioLegend (2 mentions) Streptavidin apc, by BD (2 mentions) Apc annexin 5 apoptosis detection kit with 7 aad, by BioLegend (2 mentions) Anti cd95 pe, by BD (2 mentions) Igm apc clone 2 41, by BD (2 mentions) Anti igd pe clone 11 26c 2a, by BD (2 mentions) Anti cd23 pe clone b3b4, by BD (2 mentions) Anti cd24 hsa pe, by BioLegend (2 mentions) Anti cd23 pacific blue, by BioLegend (2 mentions) Anti cd19 cf594, by BD (2 mentions)

Spelling variants of this product

Anti-CD93 (3 citations)

2 protocols using anti cd93 pe

1

Multiparametric Flow Cytometry Analysis of B Cell Subsets

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen cell suspensions or cultured B-cells were stained with the following antibodies as described:25 (link),46 (link) anti-CD138-PE (clone: 281-2); anti-CD95-PE (clone: Jo2); IgM-APC (clone II/41); anti-IgD-PE (clone 11-26c.2a); and anti-CD23-PE (clone B3B4) (all BD Pharmingen); and anti-B220-PerCP (clone: RA3-6B2); anti-CD21-APC (clone: 7E9); anti-CD24 (HSA)-PE (clone: 30-F1), anti-CD93-PE (clone: AA4.1); and anti-CD23-Pacific Blue (clone: B3B4) (all Biolegend); and anti-CD19-CF594 (clone: 1D3) (BD Horizon); and PNA-Biotin (Vector Laboratories) followed by Streptavidin-APC (BD Pharmingen). Annexin V/7-AAD stainings were performed using the APC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend). For DNA content analysis, cells were lysed and stained with propidium iodide (PI). The cells were analyzed on a FACSCalibur or a LSRII (Becton Dickinson). Transitional B-cells were identified by gating on B220+CD93+ lymphocytes.39 (link) GC B-cells were identified by gating on B220+ lymphocytes. eGFP+ and eGFP− CD138hi plasma cells were identified through the lymphocyte gate. Data were analyzed using FlowJo software.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
+ Open protocol
+ Expand
2

Multiparametric Flow Cytometry Analysis of B Cell Subsets

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen cell suspensions or cultured B-cells were stained with the following antibodies as described:25 (link),46 (link) anti-CD138-PE (clone: 281-2); anti-CD95-PE (clone: Jo2); IgM-APC (clone II/41); anti-IgD-PE (clone 11-26c.2a); and anti-CD23-PE (clone B3B4) (all BD Pharmingen); and anti-B220-PerCP (clone: RA3-6B2); anti-CD21-APC (clone: 7E9); anti-CD24 (HSA)-PE (clone: 30-F1), anti-CD93-PE (clone: AA4.1); and anti-CD23-Pacific Blue (clone: B3B4) (all Biolegend); and anti-CD19-CF594 (clone: 1D3) (BD Horizon); and PNA-Biotin (Vector Laboratories) followed by Streptavidin-APC (BD Pharmingen). Annexin V/7-AAD stainings were performed using the APC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend). For DNA content analysis, cells were lysed and stained with propidium iodide (PI). The cells were analyzed on a FACSCalibur or a LSRII (Becton Dickinson). Transitional B-cells were identified by gating on B220+CD93+ lymphocytes.39 (link) GC B-cells were identified by gating on B220+ lymphocytes. eGFP+ and eGFP− CD138hi plasma cells were identified through the lymphocyte gate. Data were analyzed using FlowJo software.
Milanovic M., Heise N., De Silva N.S., Anderson M.M., Silva K., Carette A., Orelli F., Bhagat G, & Klein U. (2016). Differential requirements for the canonical NF-κB transcription factors c-REL and RELA during the generation and activation of mature B-cells. Immunology and cell biology, 95(3), 261-271.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!