Other chemicals

Milk powder was purchased from Meiyijia supermarket; 5-HMF and FF standard were purchased from TMRM (Beijing, China). RNAiso Plus reverse transcriptase kit and fluorescence quantitative kit were purchased from Takara (Beijing, China); Primers were synthesized by Sangon Biotech (Shanghai, China). Superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) detection kit were purchased from COMIN (Suzhou, China). ROS detection kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Other chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

E. coli strains JM109 and BL21(DE3) were used as a cloning vehicle and an expression host, respectively. The EZ-10Spin Column Plasmid Mini-Prep kit and agarose gel DNA purification kit were purchased from Tiangen Co. Ltd (Beijing, China). The vectors pMD^™18-T and pET-24a(+) were purchased from Novagen (Shanghai, China). The PrimeSTAR^®HS DNA Polymerase, restriction enzymes and T4 DNA ligase were obtained from Takara (Dalian, China). Restriction endonuclease DpnI was obtained from Generay Biotech (Shanghai, China). GABA, PLP, and 1,2-phthalic dicarboxaldehyde were obtained from Sigma Chemical Co. Ltd. (Shanghai, China). Other chemicals were purchased from Sinopharm Chemical Reagent Co. Ltd. (SCRC, Shanghai, China).

PrimeSTAR polymerase, restriction enzymes, calf intestinal alkaline phosphatase, Dpn I, T4 DNA ligase, In-Fusion HD Cloning Plus kit and vector (pMD18-T) were purchased from Takara (Dalian, China). The plasmid mini-prep kit, PCR purification kit, and the agarose gel DNA purification kit were purchased from Tiangen Co. Ltd (Beijing, China). DNA sequencing and DNA primer synthesis were performed by Shanghai RuiDi Biological Technology Co. Ltd. (Shanghai, China). Tryptone and yeast extract were obtained from Oxoid (Hampshire, UK). β-cyclodextrin was purchased from Sigma-Aldrich (Milwaukee, WI, USA). Non-fat powdered milk was purchased from BBI Life Sciences (Shanghai, China). Other chemicals were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).