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Anti mouse il 2 pe

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Anti-mouse IL-2 PE is a laboratory reagent used for the detection and quantification of mouse interleukin-2 (IL-2) in biological samples. It is a conjugate of an anti-mouse IL-2 antibody and the fluorescent dye phycoerythrin (PE).

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Lab products found in correlation

Golgiplug, by BD (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions) Anti mouse ifn γ pecy7, by BD (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Anti foxp3 apc, by Thermo Fisher Scientific (1 mentions) Anti cd25 pe, by BD (1 mentions) Anti cd69 pe, by BD (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Anti icos pe, by Thermo Fisher Scientific (1 mentions) Anti cd62l pe, by Thermo Fisher Scientific (1 mentions) Macsquant analyzer, by Miltenyi Biotec (1 mentions) Anti helios pe, by Thermo Fisher Scientific (1 mentions) Anti cd4 efluor 450, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-IL-2 (30 citations) Anti-IL-2-PE (19 citations) IL-2-PE (18 citations) Anti-mouse IL-2 (9 citations) Mouse IL-2, (2 citations) Mouse anti (2 citations) PE-Cy7 anti-mouse IL-2 (2 citations)

2 protocols using anti mouse il 2 pe

1

Splenocyte Cytokine Response to HIV-1 Gag

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2×106 splenocytes were stimulated with HIV-1 Gag peptide AMQMLKETI (5 ug/ml) for 6 hours at 37°C in the presence of 1 ul/ml GolgiPlug (BD Bioscience). Cells were washed twice with FACS Buffer (PBS, 1% BSA, 0.1% sodium azide, and 1 ul/ml GolgiPlug). Splenocytes were surface stained with anti-mouse CD3 Pac Blue, anti-mouse CD4 FITC, anti-mouse CD8a PerCP for 30 minutes before being fixed with 2% paraformaldehyde and permeabilized with 0.2% saponin. Intracellular cytokines were stained with anti-mouse IL-2 PE, anti-mouse IFN-γ PEcy7, and anti-mouse TNF-Alpha APC (BD Bioscience). After 3 washings, cells were fixed with 2% formalin and run immediately on a BD LSR Fortessa cell analyzer.
Kanagavelu S., Termini J.M., Gupta S., Raffa F.N., Fuller K.A., Rivas Y., Philip S., Kornbluth R.S, & Stone G.W. (2014). HIV-1 Adenoviral Vector Vaccines Expressing Multi-Trimeric BAFF and 4-1BBL Enhance T Cell Mediated Anti-Viral Immunity. PLoS ONE, 9(2), e90100.
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2

Multiparametric Flow Cytometry Analysis

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Flow cytometry was performed on a Miltenyi Macsquant Analyzer. Antibodies used included anti-CD4 eFluor450 (eBioscience 48-0041-82), anti-CD25 PE (BD Biosciences 553866), anti-CD62L PE (eBioscience 12-0621-82), anti-CD69 PE (BD Biosciences 553237), anti-EZH2 PE (BD 562478), anti-FoxP3-APC (eBioscience 17-5773-80), anti-FoxP3-PE (eBioscience 12-5773-80), anti-Helios PE (eBioscience 12-9883-41), anti-ICOS PE (eBioscience 12-9942-81), anti-ID3 PE (BD Biosciences 564564), anti-mouse IFN gamma APC (eBioscience 17-7311-81), anti-mouse IL2 PE (BD Bioscience 561061), anti-KI67 eFluor450 (eBioscience 48-5698-80). Flow cytometry antibodies were used at a 1:100 dilution except for anti-EZH2 PE, anti-FoxP3-APC, anti-FoxP3-PE, anti-Helios PE, anti-ICOS PE, anti-ID3 PE, anti-mouse IFN gamma APC, anti-mouse IL2 PE, anti-KI67 eFluor450, which were all used at a 1:50 dilution. Cell trace violet (Invitrogen #C34557) was used following manufacturer’s specifications. Flow cytometry data analysis was performed using FlowJo.
Gerriets V.A., Kishton R.J., Johnson M.O., Cohen S., Siska P.J., Nichols A.G., Warmoes M.O., de Cubas A.A., MacIver N.J., Locasale J.W., Turka L.A., Wells A.D, & Rathmell J.C. (2016). Foxp3 and Toll-like receptor signaling balance Treg cell anabolic metabolism for suppression. Nature immunology, 17(12), 1459-1466.
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