Malvern dts v 6

The morphological characterization of MAG-EPA and MAG-EPA/citrem nanoparticles prepared using a highenergy emulsification was done under a frozen-hydrated state as previously described. 47 Briefly, 3-4 µL of the dispersions were applied on a hydrophilized lacey carbon 300 mesh copper grid (Ted Pella Inc., California, USA). The excess sample on the grid was then blotted with filter paper at blotting time 5 s, blotting force 0, temperature 25 °C and 100 % humidity (FEI Vitrobot IV, Holland), and was rapidly plunged into liquid-nitrogen cooled ethane (-180 °C). The samples observation was done with Tecnai G2 20 transmission electron microscope (FEI, Holland) at a voltage of 200 kV under low-dose rate (~5 e/Å 2 s).
Images were then recorded using a FEI Eagle camera 4x4 k at a nominal magnification of 69,000x resulting in a final image sampling of 0.22 nm/pixel. Nano ZS (Malvern Instruments, Worcestershire, U.K.) equipped with a 633 nm laser and 173° detection optics. Measurements were performed at room temperature on samples diluted 100x in water. Malvern DTS v. 6.34 software (Malvern instruments, Worcestershire, U.K.) was used for data acquisition and analysis. For the viscosity and refractive index, the values of pure water were used.

The zeta potential of the nanodispersions was measured using a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, U.K.) equipped with a 633 nm laser and 173° detection optics. Measurements were performed at room temperature on samples diluted 100x in water.
Malvern DTS v. 6.34 software (Malvern instruments, Worcestershire, U.K.) was used for data acquisition and analysis. For the viscosity and refractive index, the values of MQ water were used.