Rat specific insulin elisa kit

All chemicals in this study were supplied
from Sigma-Aldrich Co. (Gmbh, Munich,
Germany). Rat specific insulin ELISA kit was
obtained from Mercodia (Sweden). All other
chemicals which were used in the study were
obtained from standard commercial suppliers.

Serum levels of liver injury markers, including alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT)^{23 (link)}
were measured employing commercial kits (Pars Azmoon, Tehran, Iran). Fasting serum insulin concentration was determined through a rat specific insulin ELISA kit (Mercodia, Uppsala, Sweden). Moreover, fasting plasma glucose level was measured by a digital glucometer (Gluco Sure, Star, Taiwan). Then, insulin resistance was estimated by calculation of the homeostasis model assessment parameter of insulin resistance (HOMA-IR) through the following formula^{24 (link)}
:
HOMA-IR = fasting serum insulin (µU/m mL) × fasting plasma glucose (mmol/L) / 22.5