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Thermo scientific rip kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Thermo Scientific RIP Kit is a laboratory equipment used for the rapid isolation and purification of RNA-binding proteins (RBPs) from various biological samples. The kit utilizes magnetic beads coated with RBP-specific antibodies to capture and isolate RBPs from cell lysates or tissue homogenates. The isolated RBPs can then be used for further analysis, such as identification and characterization.

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Lab products found in correlation

Ab45766, by Abcam (1 mentions) Protein a g magnetic bead, by Thermo Fisher Scientific (1 mentions) Ab113205, by Abcam (1 mentions) Nf κb antibody, by Abcam (1 mentions)

2 protocols using thermo scientific rip kit

1

RNA-Protein Interactions Analysis

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RNA immunoprecipitation (RIP) assays were performed according to the instructions of the Thermo Scientific RIP Kit (Thermo, Waltham, MA, USA) using specific antibodies against HNRNPK (11426-1-AP, Proteintech, China), ILF2 (ab113205, Abcam, UK), Flag (ab45766, Abcam, UK), or IgG (30000-0-AP, Proteintech, China) and Protein A/G magnetic beads (Life Technologies, USA) for RNA immunoprecipitation. Input and coprecipitated RNA were detected via qRT‒PCR analysis.
Xi Z., Huang H., Hu J., Yu Y., Ma X., Xu M., Ming J., Li L., Zhang H., Chen H, & Huang T. (2024). LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis. Journal of Experimental & Clinical Cancer Research : CR, 43, 22.
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2

RIP Assay for LOC645166-NF-κB Interaction

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A RIP assays were carried out to study whether LOC645166 could interact with NF-κB using a Thermo Scientific RIP kit (Thermo, Waltham, MA, USA) according to the manufacturer’s instructions. NF-κB antibodies were purchased from Abcam and Rabbit IgG (Abcam) was used as the negative control. Purified RNA was subjected to RT-qPCR analysis. The sequences of each primer were shown in supplementary table 5.
Zheng R., Jia J., Guan L., Yuan H., Liu K., Liu C., Ye W., Liao Y., Lin S, & Huang O. (2020). Long noncoding RNA lnc-LOC645166 promotes adriamycin resistance via NF-κB/GATA3 axis in breast cancer. Aging (Albany NY), 12(10), 8893-8912.
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