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Thermo scientific rip kit

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Thermo Scientific RIP Kit is a laboratory equipment used for the rapid isolation and purification of RNA-binding proteins (RBPs) from various biological samples. The kit utilizes magnetic beads coated with RBP-specific antibodies to capture and isolate RBPs from cell lysates or tissue homogenates. The isolated RBPs can then be used for further analysis, such as identification and characterization.

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2 protocols using thermo scientific rip kit

1

RNA-Protein Interactions Analysis

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RNA immunoprecipitation (RIP) assays were performed according to the instructions of the Thermo Scientific RIP Kit (Thermo, Waltham, MA, USA) using specific antibodies against HNRNPK (11426-1-AP, Proteintech, China), ILF2 (ab113205, Abcam, UK), Flag (ab45766, Abcam, UK), or IgG (30000-0-AP, Proteintech, China) and Protein A/G magnetic beads (Life Technologies, USA) for RNA immunoprecipitation. Input and coprecipitated RNA were detected via qRT‒PCR analysis.
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2

RIP Assay for LOC645166-NF-κB Interaction

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A RIP assays were carried out to study whether LOC645166 could interact with NF-κB using a Thermo Scientific RIP kit (Thermo, Waltham, MA, USA) according to the manufacturer’s instructions. NF-κB antibodies were purchased from Abcam and Rabbit IgG (Abcam) was used as the negative control. Purified RNA was subjected to RT-qPCR analysis. The sequences of each primer were shown in supplementary table 5.
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