Reduced form of nicotinamide adenine dinucleotide phosphate nadph

Selol was synthesized at the Department of Bioanalysis and Drug Analysis at Medical University of Warsaw (Polish Patent 1999). A micellar solution of Selol was prepared ex tempore (based on lecithin, water, and Selol), with a declared selenium concentration of 5 % (w/v).
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), horse serum (HS), penicillin, streptomycin, l-glutamine, 3-(4,5-dimethyl-2-tiazolilo)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 2′-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazoletrihydrochloridehydrate (Hoechst 33258), TRI-reagent, polyethylenoimine (PEI), dimethyl sulfoxide (DMSO), sodium aurothiomalate (SAu), sodium selenite (Na₂SeO₃), reduced glutathione (GSH), glutathione reductase (GR), the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), sodium azide, tert-butylperoxide, 2-vinylpyridine, triethanolamine, metaphosphoric acid, bovine serum albumin (BSA), and all other common reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for reverse transcription (High Capacity RNA-to-cDNA Master Mix) and PCR (Gene Expression Master Mix) were from Applied Biosystems (Applied Biosystems, Foster City, CA, USA).