The following materials were used in the study: LPS from Escherichia coli O111:B4, Griess reagent, and Giemsa solution (all from Sigma-Aldrich, St. Louis, MO, USA); antibodies specific for TLR4, p- mitogen-activated protein kinase 7 (TAK1), TAK1, p-IKK α/β, IKK α/β, IL-1β, COX-2, p-NF-κB, NF-κB, p-ERK1/2, and ERK1/2 (all from Cell Signaling Technology, Danvers, MA, USA); antibodies specific for iRAK4, TRAF6, SOD1, GPx1, p-Raf-1, and GAPDH (all from Santa Cruz Biotechnology, Dallas, TX, USA); IL-1β, TNF-α, IFN-γ cytokine kit, PerCP/Cyanine5.5 anti-mouse Ly6G, and Alexa-647 anti-mouse CD11b (all from BioLegend, San Diego, CA, USA); and PE anti-mouse Ly6C (BD Biosciences, Becton, NJ, USA).
Percp cyanine5.5 anti mouse ly6g
Manufactured by BioLegend
Sourced in United States
PerCP/Cyanine5.5 anti-mouse Ly6G is a fluorescently conjugated antibody that binds to the Ly6G antigen expressed on the surface of mouse neutrophils. It can be used for the identification and enumeration of neutrophils in flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2, by Cell Signaling Technology (1 mentions)
Lps from escherichia coli o111 b4, by Merck Group (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Griess reagent, by Merck Group (1 mentions)
Giemsa solution, by Merck Group (1 mentions)
Cox 2, by Cell Signaling Technology (1 mentions)
Il 1β, by Cell Signaling Technology (1 mentions)
Tnf α, by BioLegend (1 mentions)
Ikkα β, by Cell Signaling Technology (1 mentions)
P raf 1, by Santa Cruz Biotechnology (1 mentions)
Gpx 1, by Santa Cruz Biotechnology (1 mentions)
Pe anti mouse ly6c, by BD (1 mentions)
P nf κb, by Cell Signaling Technology (1 mentions)
Nf κb, by Cell Signaling Technology (1 mentions)
P ikkα β, by Cell Signaling Technology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Il 1β, by BioLegend (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Pe anti mouse ly 6c, by BioLegend (1 mentions)
Apc anti mouse human cd11b, by BioLegend (1 mentions)
2 protocols using percp cyanine5.5 anti mouse ly6g
1
LPS-Induced Inflammatory Response Assay
2
Multiparametric Flow Cytometry Immunophenotyping
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The cell was labeled with FITC anti-mouse CD45 Antibody (BioLegend, No. 103107), APC anti-mouse/human CD11b (BioLegend, No. 101211), PE/cyanine seven anti-mouse F4/80 (BioLegend, No. 123113), PE anti-mouse Ly-6C (BioLegend, No. 128007), and PerCP/cyanine 5.5 anti-mouse Ly-6G (BioLegend, No. 127616) for 20 min at room temperature. Flow cytometry analysis was performed on a CytoFLEX (Beckman Coulter), and results were analyzed by FlowJo software version 10.
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