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C met sirna

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C-Met siRNA is a small interfering RNA (siRNA) designed to target the c-Met gene. The c-Met gene encodes a receptor tyrosine kinase that plays a role in cell growth, survival, and motility. C-Met siRNA can be used to study the function of the c-Met gene and its role in cellular processes.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (5 mentions) Anti β actin antibody, by Merck Group (2 mentions) E cadherin sirna, by Santa Cruz Biotechnology (2 mentions) Anti egfr antibody, by Cell Signaling Technology (2 mentions) Anti c met antibody, by Cell Signaling Technology (2 mentions) Anti e cadherin antibody, by Cell Signaling Technology (2 mentions) Control sirna, by Santa Cruz Biotechnology (1 mentions)

3 protocols using c met sirna

1

Knockdown of c-Met and E-cadherin in OKF6/TERT2 cells

Cited 1 time
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As previously described [51 (link)], OKF6/TERT2 cells were grown in 6 well plates to 80% confluency and transfected with 40 pmole of c-Met siRNA (#SC-29397, Santa Cruz Biotechnology) or E-cadherin siRNA (#SC-35242, Santa Cruz Biotechnology) using Lipofectamine 2000 (#11668027, ThermoFisher Scientific) following the manufacturer’s instructions. After 24 h, the cells were trypsinized, seeded onto fibronectin coated glass coverslips, and incubated for another 24 h before use. The extent of protein knockdown was determined by immunoblotting with an anti-c-Met antibody (#8198, Cell Signaling Technology), anti-E-cadherin antibody (#3195, Cell Signaling Technology), anti-EGFR antibody (#4267, Cell Signaling technology), and anti-β-actin antibody (#A5441, Sigma-Aldrich).
Phan Q.T., Solis N.V., Cravener M.V., Swidergall M., Lin J., Huang M.Y., Liu H., Singh S., Ibrahim A.S., Mazzone M., Mitchell A.P, & Filler S.G. (2023). Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection. PLOS Pathogens, 19(8), e1011579.
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2

Knockdown of c-Met and E-cadherin in OKF6/TERT2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously described (Phan et al., 2021 (link)), OKF6/TERT2 cells were grown in 6 well plates to 80% confluency and transfected with 40 pmole of c-Met siRNA (#SC-29397, Santa Cruz Biotechnology) or E-cadherin siRNA (#SC-35242, Santa Cruz Biotechnology) using Lipofectamine 2000 (#11668027, ThermoFisher Scientific) following the manufacturer’s instructions. After 24 h, the cells were trypsinized, seeded onto fibronectin coated glass coverslips, and incubated for another 24 h before use. The extent of protein knockdown was determined by immunoblotting with an anti-c-Met antibody (#8198, Cell Signaling Technology), anti-E-cadherin antibody (#3195, Cell Signaling Technology), anti-EGFR antibody (#4267, Cell Signaling technology), and anti-β-actin antibody (#A5441, Sigma-Aldrich).
Phan Q.T., Solis N.V., Cravener M.V., Swidergall M., Lin J., Huang M.Y., Liu H., Singh S., Ibrahim A.S., Mazzone M., Mitchell A.P, & Filler S.G. (2023). Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection. bioRxiv.
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3

Transient c-Met Silencing in Cells

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Cells were transfected with either pooled c-Met siRNA (100 nM) (#sc-29397, pooled of three transcripts, Santa Cruz Biotech. Inc., Dallas, TX) or control siRNA (100 nM) (Santa Cruz proprietary control sequence based upon (13 (link)), Santa Cruz Biotech, Inc.) using Lipofectamine-2000 (Invitrogen, Carlsbad, CA) for 4 h.
Kumar D., New J., Vishwakarma V., Joshi R., Enders J., Lin F., Dasari S., Gutierrez W.R., Leef G., Ponnurangam S., Chavan H., Ganaden L., Thornton M.M., Dai H., Tawfik O., Straub J., Shnayder Y., Kakarala K., Tsue T.T., Girod D.A., Van Houten B., Anant S., Krishnamurthy P, & Thomas S.M. (2018). Cancer-associated fibroblasts drive glycolysis in a targetable signaling loop implicated in head and neck squamous cell carcinoma progression. Cancer research, 78(14), 3769-3782.
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