Cells (1×105) were plated in IF dishes (NEST Biotechnology, Wuxi, China) and treated with DIOS. Then, the cells were fixed in paraformaldehyde and permeabilized with 0.01% Triton X-100. Next, the cells were incubated with primary antibodies against p53 (#2527) and Notch3 (#5276) [1:500 dilution with 5% bovine serum albumin (BSA) in PBS; Cell Signaling Technology, Inc.] at 4°C overnight upon blocking the cells with 5% BSA dilution in PBS. DyLight™ 649-conjugated secondary antibodies (#009-490-006; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) were applied at a dilution of 1:500 at room temperature for 1 h. The specimens were monitored using a TCP SP5 laser microscope (Leica Microsystems GmbH, Wetzlar, Germany).
Dylight 649 conjugated secondary antibodies
Manufactured by Jackson ImmunoResearch
Sourced in United States
DyLight™ 649-conjugated secondary antibodies are fluorophore-labeled immunoglobulin detection reagents. They are designed to bind to primary antibodies of various host species, allowing for highly sensitive detection in immunoassays and other immunolabeling applications.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Cell Signaling Technology (1 mentions)
Alexafluor 488 conjugated secondary antibody, by Merck Group (1 mentions)
Rhodamine pna, by Vector Laboratories (1 mentions)
Cy3 conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Mouse anti ctbp2, by BD (1 mentions)
Mouse anti s100b, by Merck Group (1 mentions)
Rabbit anti m opsin, by Merck Group (1 mentions)
Rabbit anti pikachurin, by Fujifilm (1 mentions)
Goat anti s opsin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using dylight 649 conjugated secondary antibodies
1
Immunofluorescence of p53 and Notch3
2
Immunostaining of Retinal Cell Markers
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We used the following primary antibodies for immunostaining: mouse anti-Cacna1s (1:250, Millipore), rabbit anticalbindin (1:1000, Calbiochem), mouse anti-Ctbp2 (1:500, BD Biosciences), rabbit anti-M-opsin (1:500, Millipore), guinea pig anti-mGluR6 (1:500) (Koike et al. 2010) , mouse anti-PKC-a (1:500, Upstate), rhodamine-PNA (1:250, Vector Laboratories), rabbit anti-pikachurin (1:250, Wako) (Sato et al. 2008) , rabbit anti-rhodopsin (1:5000, Sigma), mouse anti-S100b (1:2500, Sigma), goat anti-S-opsin (1:500, Santa Cruz), mouse anti-ROM1 (1:100, a gift from Dr. R. Molday, the University of British Columbia, Canada) and rabbit anti-Trpm1 (1:100) (Koike et al. 2010) . We used Cy3-conjugated secondary antibodies (1:500, Jackson ImmunoResearch Laboratories), Alexa Fluor 488-conjugated secondary antibodies (1:500, Sigma) and DyLight 649-conjugated secondary antibodies (1:500, Jackson ImmunoResearch Laboratories).
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