Uridine-5-triphosphate (UTP; cat #6701) and phorbol 12,13-dibutyrate (PDBu; cat #524390) were purchased from Calbiochem. Calyculin A (cat #9902) was purchased from Cell Signaling Technology. The anti-HA antibody (cat #11867423001) was purchased from Roche. The anti-phospho-PKCα/βII turn motif (pThr638/641; cat #9375) was from Cell Signaling Technology. Lipids used in kinase assays (DG, cat #800811C and PS, cat #840034C) were purchased from Avanti Polar Lipids. The anti-phospho-PKCγ hydrophobic motif (pThr674; cat# ab5797) antibody was from Abcam. The anti-phospho-PKCα/β/γ activation loop (pThr497/500/514) antibody was previously described (44 (link)). Ladder (cat #161-0394), bis/acrylamide solution (cat # 161–0156), and PVDF (cat# 162–0177) used for SDS-PAGE and Western blotting were purchased from Bio-Rad. Luminol (cat #A-8511) and p-coumaric acid (cat # C-9008) used to make chemiluminescence solution for western blotting were purchased from Sigma-Aldrich. [γ−32P]-ATP (cat # BLU002Z500UC) was purchased from Perkin Elmer. Phosphatidylserine (cat # 840034C) and diacylglycerol (cat # 800811C) were from Avanti Polar Lipids. The PKC substrate peptide (Ac-FKKSFKL-NH2) was synthesized by GenScript.
Anti phospho pkcα βii turn motif pthr638 641
Manufactured by Cell Signaling Technology
Anti-phospho-PKCα/βII turn motif (pThr638/641) is a laboratory reagent used for the detection and quantification of phosphorylation of the turn motif of Protein Kinase C (PKC) α and βII isoforms at Thr638 and Thr641, respectively. This reagent is designed for use in techniques such as Western blotting, immunohistochemistry, and ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Calyculin a, by Cell Signaling Technology (2 mentions)
Anti phospho pkcγ hydrophobic motif pthr674, by Abcam (2 mentions)
Acrylamide bis solution, by Bio-Rad (2 mentions)
Anti ha antibody, by Roche (2 mentions)
Polyvinylidene fluoride (pvdf), by Bio-Rad (2 mentions)
Luminol, by Merck Group (2 mentions)
P coumaric acid, by Merck Group (2 mentions)
Phosphatidylserine, by Avanti Polar Lipids (1 mentions)
Diacylglycerol, by Avanti Polar Lipids (1 mentions)
γ 32p atp, by PerkinElmer (1 mentions)
2 protocols using anti phospho pkcα βii turn motif pthr638 641
1
Comprehensive PKC Assay Protocol
2
Kinase Assay and Western Blotting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Uridine-5-triphosphate (UTP; cat #6701) and phorbol 12,13-dibutyrate (PDBu; cat #524390) were purchased from Calbiochem. Calyculin A (cat #9902) was purchased from Cell Signaling Technology. The anti-HA antibody (cat #11867423001) was purchased from Roche. The antiphospho-PKCα/βII turn motif (pThr638/641; cat #9375) was from Cell Signaling Technology.
Lipids used in kinase assays (DG, cat #800811C and PS, cat #840034C) were purchased from Avanti Polar Lipids. The anti-phospho-PKCγ hydrophobic motif (pThr674; cat# ab5797) antibody was from Abcam. The anti-phospho-PKCα/β/γ activation loop (pThr497/500/514) antibody was previously described (44) . Ladder (cat #161-0394), bis/acrylamide solution (cat # 161-0156), and PVDF (cat# 162-0177) used for SDS-PAGE and Western blotting were purchased from Bio-Rad.
Luminol (cat #A-8511) and p-coumaric acid (cat # C-9008) used to make chemiluminescence solution for western blotting were purchased from Sigma-Aldrich.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Lipids used in kinase assays (DG, cat #800811C and PS, cat #840034C) were purchased from Avanti Polar Lipids. The anti-phospho-PKCγ hydrophobic motif (pThr674; cat# ab5797) antibody was from Abcam. The anti-phospho-PKCα/β/γ activation loop (pThr497/500/514) antibody was previously described (44) . Ladder (cat #161-0394), bis/acrylamide solution (cat # 161-0156), and PVDF (cat# 162-0177) used for SDS-PAGE and Western blotting were purchased from Bio-Rad.
Luminol (cat #A-8511) and p-coumaric acid (cat # C-9008) used to make chemiluminescence solution for western blotting were purchased from Sigma-Aldrich.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
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