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Anti lubricin

Manufactured by Abcam

Anti-Lubricin is a laboratory reagent used to detect and quantify the presence of lubricin, a glycoprotein that plays a critical role in joint lubrication and cartilage protection. This product allows researchers to measure lubricin levels in various biological samples, providing valuable insights into joint health and disease processes.

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2 protocols using anti lubricin

1

Reagents for Lubrication Testing

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Reagents for lubrication testing materials were as described previously,2 (link),6 (link),33 (link) including hyaluronan (HA) in a 4,000 kDa form (Healon®, Advanced Medical Optics, Santa Ana, CA). The antibody to PRG4 was anti-Lubricin from AbCam (Cambridge, MA);2 (link) nonspecific rabbit IgG was from Pierce (Rockford, IL); mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch (West Grove, PA). Streptomyces hyaluronidase was from Seikagaku (Tokyo, Japan). SeaKem® gold agarose was from Lonza (Rockland, ME); 50X TAE (2M Tris, 0.5M EDTA) electrophoresis buffer was from Life Technologies (Carlsbad, CA); Hybond™-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare (Piscataway, NJ). Stains-All was from Sigma-Aldrich (St. Louis, MO).
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2

Lubrication Testing Materials Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents for lubrication testing materials were as described previously,2 (link),6 (link),33 (link) including HA in a 4000-kDa form (Healon; Advanced Medical Optics). The antibody to PRG4 was anti-Lubricin from AbCam,2 (link) nonspecific rabbit IgG was from Pierce, and mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch. Streptomyceshyaluronidase was from Seikagaku, SeaKem gold agarose was from Lonza; 50X TAE (2M Tris, 0.5 M ethylenediamine tetraacetic acid [EDTA]) electrophoresis buffer was from Life Technologies, Hybond-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare, and Stains-All was from Sigma-Aldrich.
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