Mirneasy serum plasma micro kit

Circulating miRNAs were isolated from serum samples obtained from groups F and G using the miRNeasy serum/plasma micro kit (Qiagen). RNA concentration and quality were measured and verified using a NanoPhotometer N60 spectrophotometer (Implen NanoPhotometer). For normalizing circulating miRNA expression, synthetic Caenorhabditis elegans miRNA-39 (cel-miR-39, Applied Biosystems) was added to the serum samples, as described previously [36 (link)] (Figure 5).

The miRNeasy serum/plasma micro kit (Qiagen) was used for total RNAs isolation. The synthetic Caenorhabditis elegans miRNA (cel‐miR‐39; Applied Biosystems) was added to each sample as external reference.
A multiphase case‐control study was designed to identify the exosome‐derived lncRNA profile as a signature for HCC.
In the screening stage, RNA extracted from three HCC patients without metastasis (HCC‐N), three HCC patients with metastasis (HCC‐M), three patients diagnosed with chronic hepatitis (HCC) and three paired controls samples were also subjected for Human LncRNA Array v3.0 (Arraystar; Agilent) to screen the lncRNAs in different groups. Based on this, the RT‐qPCR was firstly used in the training set to further filter signals of the screened lncRNAs. The detailed steps for candidate screening and data filtering parameters were presented in Figure S1. The detailed primer sequence has been listed in Table S6.