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Hippocalcin

Manufactured by Abcam
Sourced in United States

Hippocalcin is a calcium-binding protein that plays a role in regulating various cellular processes. It is expressed in the hippocampus and other brain regions. Hippocalcin is involved in calcium signaling and may contribute to neuronal plasticity and function.

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2 protocols using hippocalcin

1

Biochemical and Immunological Reagents for Research

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T4 DNA ligase, NdeI, and EcoRI were obtained from New England Biolabs (Ipswich, MA, USA). Alexa Fluor 488- and 594-conjugated donkey secondary antibodies were obtained from Invitrogen (Carlsbad, CA, USA). Streptozotocin (STZ), D-glucose, and some reagents for the western blot analysis such as mouse anti-glial fibrillary acidic protein (GFAP), β-actin, and α-tubulin were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Primary antibodies such as GLP-1, Tau, NF-κBp65, insulin receptor (IR)-β, and calbindin were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA). Rabbit anti-parvalbumin, GLP-1R, hippocalcin, nuclear p84, and mitochondrial VDAC1 were obtained from Abcam (Cambridge, MA, USA). Isopropyl-β-thiogalactopyranoside (IPTG), LB broth, p-Tau, and 4′,6-diamidino-2-phenylindol (DAPI) were obtained from the Thermo Fisher Scientific (Waltham, MA, USA). Total dynamin related protein1 (Drp1) and optic atrophy1 (OPA) were obtained from BD Bioscience (Franklin Lakes, NJ, USA).
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2

NMNAT1 Regulation of Mitochondrial Function

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Salvianolic acid A (SML0045) was purchased from Sigma. High glucose DMEM (10569010), Lipofectamine 3000 Transfection Reagent (1L3000150), fetal bovine serum (FBS) (10099), G418 (10131027), and puromycin (A1113803) were purchased from Thermo Fisher. Total RNA Extraction kit (R1200), MTT reagent (M8180), and TaqMan One Step RT-qPCR Kit (T2210) were purchased from Solarbio. Quick Ligase (M2200S), HindIII (R3104S), and EcoRI (R3101S) were purchased from NEB. Esp3I (ER0452) was purchased from Thermo. Anti-Anti-NMNAT1 (ab45652, 30), PINK1 (ab23707, 66), MAVS (ab189303, 57), N-methyl-D-aspartate receptor (NMDAR) (ab134308, 105), CaMKI (ab68234, 41), TRAF6 (ab227560, 58), p-JNK (ab239886, 48) , JNK (ab208035, 48), DLK2 (ab171037, 45), p-MKK4 (ab52958, 44), MKK4 (ab33912, 44), MLK3 (ab51068, 93), Hippocalcin (ab24560, 23), SCG10 (ab115513, 21) , and ASC (ab155970) antibodies were purchased from Abcam. NAD/NADH Assay (ab65348), Calcium Assay Kit (ab102505), and Complex I Rodent Profiling ELISA Kit (ab136809) were obtained from Abcam. ROS detection kits (E004-1-1) were purchased from Nanjing Jiancheng Bioengineering Institute. Annexin V-FITC/PI kit (CA1020) was purchased from Solarbio.
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