Live dead baclight viability stain kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The LIVE/DEAD BacLight viability stain kit is a fluorescent staining solution used to determine the viability of bacterial cells. It contains two nucleic acid-binding stains: SYTO 9, which labels live cells, and propidium iodide, which labels dead or membrane-compromised cells. This kit allows for the rapid and easy differentiation between live and dead bacterial cells through fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Las software, by Leica (1 mentions) 2000fx tem, by JEOL (1 mentions)

Close spelling variants of this product

LIVE/DEAD BacLight stain Live/Dead viability stain LIVE/DEAD BacLight LIVE/DEAD stain Viability stain

2 protocols using live dead baclight viability stain kit

Microscopic Examination of Anode Biofilms

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Cells were examined with transmission electron microscopy by placing anode-grown cells on 400-mesh carbon-coated copper grids. After 4 min, to facilitate adsorption of cells to the grid, the grids were negatively stained with 2% uranyl acetate. Samples were examined with a JEOL 2000fx transmission electron microscope operated at 200-kV accelerating voltage.
Anode biofilms were imaged with confocal laser scanning microscopy using the LIVE/DEAD BacLight viability stain kit from Molecular Probes (Eugene, OR) as previously described (34 (link)).

Tan Y., Adhikari R.Y., Malvankar N.S., Ward J.E., Woodard T.L., Nevin K.P, & Lovley D.R. (2017). Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity. mBio, 8(1), e02203-16.

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Visualizing Anode Biofilm Structure

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For the confocal laser scanning microscopy, the anode biofilms were imaged with LIVE/DEAD BacLight viability stain kit from Molecular Probes (Eugene, OR, USA) as previously described (Franks et al., 2010 (link); Nevin et al., 2011 (link)). Images were processed and analyzed using the Leica LAS software (Leica). For transmission electron microscopy cells from the anode biofilms were directly placed on copper grids coated with carbon and absorbed for 4 min. The cells were negatively stained with 0.2% uranyl acetate and examined with a JEOL 2000fxTEM at a 200 kV accelerating voltage.

Tan Y., Adhikari R.Y., Malvankar N.S., Ward J.E., Nevin K.P., Woodard T.L., Smith J.A., Snoeyenbos-West O.L., Franks A.E., Tuominen M.T, & Lovley D.R. (2016). The Low Conductivity of Geobacter uraniireducens Pili Suggests a Diversity of Extracellular Electron Transfer Mechanisms in the Genus Geobacter. Frontiers in Microbiology, 7, 980.

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