Antibodies that recognize pAKT (Ser473) (Cell Signaling Technology, #9271), AKT (Cell Signaling Technology, #9272), pERK (Thr202/Tyr204) (Cell Signaling Technology, #4370), ERK (Cell Signaling Technology, #4695), p85α (Santa Cruz, sc-376112), p110α (Cell Signaling Technology, #4249), β-actin (Sigma, A1978), NCAD (Abcam, AB98952), ECAD (Cell Signaling Technology, #3195), VIM (RV202) (Abcam, AB8978), ZEB1 (D80D3) (Cell Signaling Technology, #3396), pGSK3β (Ser9) (Cell Signaling Technology, #9323), GSK3β (Y174) (Abcam, AB32391), and non-phospho CTNNB1 (Ser33/37/Thr41) (Cell Signaling Technology, #4270) were used in the study. Horseradish peroxidase (HRP) labelled secondary antibody (Beyotime Biotech) was used in western blots. Protein A-Sepharose 4B beads (Invitrogen, 10-1041) was used for immunoprecipitation studies.
Gsk3β y174
Manufactured by Abcam
Sourced in United States
GSK3β (Y174) is a recombinant protein that corresponds to a portion of the glycogen synthase kinase 3 beta (GSK3β) protein. GSK3β is a serine/threonine protein kinase involved in the regulation of various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Merck Group (2 mentions)
P gsk3β ser9, by Cell Signaling Technology (1 mentions)
P erk thr202 tyr204, by Cell Signaling Technology (1 mentions)
Protein a sepharose 4b beads, by Thermo Fisher Scientific (1 mentions)
E cad, by Cell Signaling Technology (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
N cad, by Abcam (1 mentions)
Horseradish peroxidase hrp labelled secondary antibody, by Beyotime (1 mentions)
Zeb1 d80d3, by Cell Signaling Technology (1 mentions)
Ab75745, by Abcam (1 mentions)
Protease inhibitor cocktail tablets, by Abcam (1 mentions)
Bradford assay, by Bio-Rad (1 mentions)
Bsa standard, by Merck Group (1 mentions)
P53 do 1, by Santa Cruz Biotechnology (1 mentions)
2 protocols using gsk3β y174
1
Cell Signaling Pathway Antibodies
2
Western Blot Analysis of Protein Signaling
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Western blotting was performed as described in [25] . Briefly, cells were washed with ice-cold PBS and soluble proteins were extracted with cell lysis buffer (100 mM Tris-HCl pH=8, 150 mM NaCl, 1% NP-40, phosphatase and protease inhibitor cocktail tablets (Abcam) according to the manufacturer`s protocol). The protein concentration was determined using the Bio-Rad Bradford assay (Bio-Rad, Hercules, CA) and BSA standards (Sigma Aldrich). An equal amount of protein was separated by SDS-PAGE. The following antibodies were used: p53 (DO-1, Santa Cruz, St Jose, CA, USA), GSK3α (EP793Y, Abcam), GSK3β (Y174, Abcam), p-GSK3 (α/β) (Tyr279/Tyr216) (ab75745, Abcam), p-GSK3α (Ser21) (ab28808, Abcam), p-GSK3β (Ser9) (EPR2286Y, Abcam), and β-actin (Sigma Aldrich).
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