Igm fc antibody hrp igm

IC28 peptide with a cysteine residue at the C-terminus was synthesized by GL Biochem Co. (Shanghai, China). Human angiotensin-converting enzyme 2 (hACE2), hACE2-specific rabbit antibody, and HRP-conjugated goat antirabbit IgG antibody were ordered from Sino Biological (Beijing, China). Mannan from S. cerevisiae, 1-anilino-8-naphthalene sulfonic acid (ANS), 6-maleimidohexanoic acid N-hydroxysuccinimide ester (EMCS), sodium cyanoborohydride, dithio-bis-(2-nitrobenzoic acid (DTNB) and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Sigma (USA). Horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG Fc antibody (HRP-IgG), IgG1 Fc antibody (HRP-IgG1), IgG2a Fc antibody (HRP-IgG2a) and IgM Fc antibody (HRP-IgM) were purchased from Abcam (USA). IFN-γ ELISA kit was ordered from Biolegend (USA). IL-5 and IL-10 ELISA kits were purchased from Invitrogen (USA).

Eight-arm PEG N-hydroxysuccinimide ester with an Mw of 10 kDa (8-arm PEG), 6-maleimidohexanoic acid N-hydroxysuccinimide ester (EMCS), iminothiolane hydrochloride (IT), and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich (USA). CRM₁₉₇ was kindly provided by Hualan Biological Engineering, Inc. (China). The RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco (USA). Horseradish peroxidase-conjugated goat anti-mouse IgG Fc antibody (HRP-IgG), IgG1 Fc antibody (HRP-IgG1), IgG2a Fc antibody (HRP-IgG2a) and IgM Fc antibody (HRP-IgM) were ordered from Abcam (USA). IFN-γ and IFN-β ELISA kits were ordered from Legend (USA). TNF-α and IL-5 ELISA kits were ordered from Invitrogen (USA). All other reagents were of analytical grade.