In Chronic DSS and CAC experiments, faeces were collected from each animal on 3 occasions; (1) before DSS start/1 week after AOM injection (W5); (2) one week post-DSS removal during 1x DSS cycle (W7); and (3) at the end of the study (W15). Caecal content from the acute colitis study was collected at the end of the study. Faeces and caecal contents were used for 16S rRNA sequencing. Fat and lean body mass were measured as described using a Minispec mq benchtop NMR spectrometer (Bruker Instruments, Rheistetten, Germany) [59 (link)]. At the end of the experiments, blood (cytokines/metabolic markers), spleen, and MLNs cells (immune phenotyping), liver (free fatty acids, FFA), caecum content (SCFAs), and colon-tumour bearing (tumour+) and non-tumour bearing tissue (tumour-) [RNA, protein and histology, and immunohistochemistry] were collected.
Minispec mq benchtop nmr spectrometer
Manufactured by Bruker
Sourced in Germany
The Minispec mq benchtop NMR spectrometer is a compact and versatile nuclear magnetic resonance (NMR) instrument designed for routine analysis. It provides rapid, non-destructive measurements of various sample properties.
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2 protocols using minispec mq benchtop nmr spectrometer
1
Multimodal Analysis of Colitis-Associated Colorectal Cancer
2
Dietary n-3 Fatty Acid Effects on Murine Metabolism
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Male and female C57BL/6J mice, eight-week old, were purchased from Harlan Laboratories, UK. Animals were kept in the University College Cork animal facility on a 12 h light-12 h dark cycle at 20 ± 1°C. After acclimatisation, female and male breeding pairs were combined and assigned to one of three diets:control (CON), n-3-supplemented (n3+) diet and n-3-deficient (n3-) dietfrom gestational day 0. Male offspring (n 10/group) were randomised within treatment groups, grouphoused (3-4/cage) and weaned on to the same diets as their mothers.
Food intake and body weight were recorded weekly during 12 weeks of dietary treatment. Fat and lean body mass were measured using a Minispec mq bench-top NMR spectrometer (Bruker Instruments) before euthanasia. The welfare and general health status of the animals were continuously monitored throughout the experimental period. No adverse events were observed. All experiments were conducted in accordance with the Directive 2010/63/EU and were approved by the Animal Experimentation Ethics Committee of University College Cork no. 2012/036.
Food intake and body weight were recorded weekly during 12 weeks of dietary treatment. Fat and lean body mass were measured using a Minispec mq bench-top NMR spectrometer (Bruker Instruments) before euthanasia. The welfare and general health status of the animals were continuously monitored throughout the experimental period. No adverse events were observed. All experiments were conducted in accordance with the Directive 2010/63/EU and were approved by the Animal Experimentation Ethics Committee of University College Cork no. 2012/036.
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