Serum urea and creatinine were measured with spectrophotometric reagents commercially available (MAK006-1KT, MAK080-1KT, Sigma, Saint-Louis, MO, USA) and the absorbance was measured using Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA). Blood urea nitrogen (BUN) was calculated using the following formula: urea (mg/dL) × 0.467 = BUN (mg/dL).
Mak006 1kt
Manufactured by Merck Group
Sourced in United States
MAK006-1KT is a laboratory equipment product from Merck Group. It is used for a specific analytical function, but without further details on its intended use or interpretation of its capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Mak080 1kt, by Merck Group (2 mentions)
Multiskan fc microplate photometer, by Thermo Fisher Scientific (1 mentions)
Ab119602, by Abcam (1 mentions)
Ab119597, by Abcam (1 mentions)
Multiscan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Mak055 1kt, by Merck Group (1 mentions)
Mak052 1kt, by Merck Group (1 mentions)
4 protocols using mak006 1kt
1
Spectrophotometric Determination of Serum Urea and Creatinine
2
Hepatocyte Co-Culture Ammonia Metabolism
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Sixteen to twenty hours after seeding hepatocytes, culture media was removed from bioreactors and 2 L of co-culture media supplemented with 0.8 mM ammonium chloride. Bioreactor media perfusion was resumed, and media samples were collected in duplicate at t = 0 h, 1 h, 2 h, 7 h, and 23 h. Media ammonia levels were quantified on a CEDEX BioHT, and duplicate frozen samples were assayed in parallel to measure urea produced over time (Sigma, MAK0061KT).
3
Serum Biomarkers for Kidney Injury
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Blood biochemical analysis was performed according to previous investigations. The blood samples were extracted 24 h following sham or CLP operation and centrifuged at 1400 x g to prepare serum at 4°C for 10 min. SCr levels were evaluated by a creatinine enzymatic assay kit (MAK080-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. BUN was detected with an enzymatic assay kit (MAK006-1KT, Sigma-Aldrich, Merck KGaA) and measured at 570 nm. The expression levels of NGAL (ab119602, Abcam) and KIM-1(ab119597, Abcam) were measured using corresponding kits and measured at 450 nm. All measurements were conducted with a multiscan spectrum spectrophotometer (Thermo Fisher Scientific Inc.)
4
Serum Biomarker Analysis in Tumor-Bearing Mice
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Mouse blood was collected without using an anticoagulant and was allowed to clot for 2 h at room temperature. The serum was then separated by centrifugation at 2000 g for 15 min. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and blood urea nitrogen (BUN) in serum from tumor-bearing mice were analyzed using the commercially available kits (Cat# MAK052-1KT, MAK055-1KT and MAK006-1KT, Sigma-Aldrich, St. Louis, MO, USA).
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