Gallic acid, paeonol, and paeoniflorin were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Dexamethasone (Dex) was obtained from Sigma Chemical Co. (St Louis, MO, USA). HPLC-grade formic acid was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). All the above chemical reagents were HPLC≥98%. Acetonitrile hypergrade for UPLC-Q/TOF-MS was acquired from Merck KGaA (Darmstadt, Germany). The polyethylenimine (PEI) transient transfection reagent was purchased from PolyScience (Carlsbad, CA, USA). Dual-Luciferase® Reporter Assay System 10-Pack was obtained from Promega (Madison, WI, USA). Both interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) Human ELISA Kit were purchase from Abcam (Cambridge, UK). All reagents for cell culture were obtained from biological industries (Israel).
Paeoniflorin
Manufactured by Maclin Biochemical Technology
Sourced in China
Paeoniflorin is a key bioactive compound extracted from the roots of the Paeonia plant species. It is a white crystalline powder with a molecular formula of C23H28O11. Paeoniflorin is commonly used as a reference standard in analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Maclin Biochemical Technology (2 mentions)
Paeonol, by Maclin Biochemical Technology (1 mentions)
Dual luciferase reporter assay system 10 pack, by Promega (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Tannic acid, by Maclin Biochemical Technology (1 mentions)
D glucose, by Maclin Biochemical Technology (1 mentions)
Dl2000 plus dna marker, by Vazyme (1 mentions)
Sybr green premix pro taq hs qpcr kit, by Accurate Biology (1 mentions)
Evo m mlv rt premix, by Accurate Biology (1 mentions)
3 protocols using paeoniflorin
1
Phytochemical-Mediated Anti-Inflammatory Regulation
2
Characterization of Paeoniae Radix
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RPA was purchased from Yonggang Decoction Piece Factory Co., Ltd. (Bozhou, China). The standard substances including paeoniflorin, benzoylpaeoniflorin, gallic acid, tannic acid, 2′-hydroxy-4′-mehoxyacetophenone, and D-(+)-glucose were HPLC grade and bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). The water used in this study was deionized and prepared by the PSDK-C system (Beijing, China).
3
Paeoniflorin and Rifaximin Protocol
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Paeoniflorin (purity ≥98%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China; lot no. C12198153). Rifaximin was purchased from Alfasigma Pharmaceutical (Alanno, Italy; lot no. 22953). EVO M-MLV RT Premix and the SYBR® Green Premix Pro Taq HS qPCR kit for qPCR were obtained from Accurate Biotechnology Co., Ltd. (Changsha, China). The DL2000 Plus DNA Marker and DNA loading buffer were obtained from Vazyme Biotech Co. Ltd. (Nanjing, China). Primers for mmu-miR-29a-3p were synthesized by Accurate Biotechnology Co., Ltd. Primers for NLRP3, ASC, caspase-1, IL-1β, TNF-α, and β-actin were synthesized by Shanghai Generay Biotech Co. Ltd. (Shanghai, China).
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