For NMR spectroscopy (1H 400MHz, 13C 100MHz, JEOL JNMECP 400 NMR spectrometer), chloroform-D (Cambridge Isotope Laboratories, Inc., USA) was used as the solvent. Human liver hepatocellular carcinoma cell line (HepG2) was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Cell culture media Dulbecco’s Modified Eagle Medium (DMEM), penicillin/streptomycin, fetal bovine serum (FBS) were purchased from Gibco BRL, Life Technology (NY, USA). 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was purchased from Sigma Chemical Co. (St. Louis, MO, USA). All antibodies used for Western blot analysis were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Other chemicals and reagents used of analytical grade commercially available.
3 4 5 dimethyl 2 yl 2 5 diphenyltetrazolium bromide mtt reagent
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent is a colorimetric assay used to measure cell metabolic activity. It is a yellow tetrazolium salt that is reduced to a purple formazan product by metabolically active cells.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform d, by Cambridge Isotopes (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
α mem, by Thermo Fisher Scientific (1 mentions)
P nitrophenyl phosphate pnpp substrate, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
β glycerol phosphate, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 protocols using 3 4 5 dimethyl 2 yl 2 5 diphenyltetrazolium bromide mtt reagent
1
NMR Spectroscopy Protocol for HepG2 Cell Analysis
2
Osteoblast Differentiation of S. glabra Roxb
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The roots of S. glabra Roxb were collected in Thai Nguyen province, Vietnam in September 2016 and identified by Prof. Phan Ke Loc. The voucher specimen (SGR92016) was deposited at the Institute of Ecology and Biological Resource, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
The osteoblast cells MC3T3-E1 and human mesenchymal stem cells (MSCs) were obtained from American Type of Culture Collection (Manassas, VA, USA). Minimum essential medium α (MEM α) was obtained from Gibco BRL, Life Technologies (USA). 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent, trypsin-EDTA, penicillin/streptomycin, p-nitrophenyl phosphate (pNPP) substrate, ascorbic acid, dexamethasone, βglycerol phosphate, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals and reagents were of analytical grade.
The osteoblast cells MC3T3-E1 and human mesenchymal stem cells (MSCs) were obtained from American Type of Culture Collection (Manassas, VA, USA). Minimum essential medium α (MEM α) was obtained from Gibco BRL, Life Technologies (USA). 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent, trypsin-EDTA, penicillin/streptomycin, p-nitrophenyl phosphate (pNPP) substrate, ascorbic acid, dexamethasone, βglycerol phosphate, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals and reagents were of analytical grade.
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