Histone h3 antibody

Protein extracts were resolved by SDS-PAGE, and indicated primary antibodies were used. For PAR analysis, cells were first treated with indicated doses of olaparib for 30 minutes prior to harvest. For PARP trapping analysis, cells were first treated with 0.01% MMS and indicated doses of olaparib for 4 hours prior to harvest. Antibodies used were as follows: ABCB1 antibody (SC-8313, rabbit-polyclonal, 1:500 dilution) was purchased from Santa Cruz Biotechnology; PARP antibody (CS-9532S, rabbit-monoclonal antibody, 1:1000 dilution) was purchased from Cell Signaling Technology; PAR antibody (4335-MC-100, mouse-monoclonal antibody, 1:1000 dilution) was purchased from Trevigen; Tubulin antibody (T5168, mouse monoclonal antibody, 1:6000 dilution) was purchased from Sigma-Aldrich; GAPDH antibody (MAB374, mouse monoclonal antibody, 1:10000) was purchased from EMD Millipore; and Histone-H3 antibody (cat. #39163) was purchased from Active Motif. Tubulin and Histone-H3 were used to monitor the amounts of samples applied. Proteins were visualized with a chemiluminescence detection system (cat. #WBLUR0500) purchased from Millipore. Densitometry to quantify blots was performed using ImageJ.