The sequences were analyzed using the Lasergene package version 10 (DNASTAR, Inc., USA) and deposited in the GenBank database under the following accession numbers: vaccine A, OM685193; vaccine B, OM685194; vaccine B_single passage, OM685195; vaccine C, OM685196. The nucleotide sequences were aligned by the Clustal W method using the DNASTAR 2.0 MegAlign program, and a phylogenetic tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates using MEGA X software (version 10.2.2). For comparison, the nucleotide sequences of the S genes of the following strains were downloaded from the NCBI GenBank database: Conn48725 (FJ899692.1), Beaudette (M95169.1), M41 (AY561711.1), H120 (FJ888351.1), K046-12 (MK618758.1), BP-CaKII (MF924724.1), QXIBV (AF193423.1), K40/09 (HM486957.1), K047-12 (MK618759.1), ArkDPI (AF006624.1), GX2-98 (AY251816.1), K210-02 (AY257068.1), and Kr/D85/06 (EF621400.1). Chromatograms were reviewed manually using BioEdit version 7.2.5 to detect the presence of viral subpopulations in the vaccines.
Lasergene package version 10
Manufactured by DNASTAR
Sourced in United States
Lasergene is a software package that provides comprehensive tools for DNA and protein sequence analysis, including sequence assembly, alignment, and visualization. The core function of Lasergene package version 10 is to facilitate the analysis and management of genetic data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lasergene package version 10
1
Comparative Genomic Analysis of Avian Coronavirus Vaccines
2
Phylogenetic Analysis of SARS-CoV-2 Vaccine Variants
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The sequences received from Macrogen were analyzed using the Lasergene package version 10 (DNASTAR, Inc., USA) and deposited in GenBank as follows: Vaccine A, OM685193; Vaccine B, OM685194; Vaccine B_single passage, OM685195; Vaccine C, OM685196. The nucleotide sequences were aligned via the Clustal W method using the DNASTAR 2.0 MegAlign program and the phylogenetic tree was constructed via the neighbor-joining method with 1,000 bootstrap replicates using MEGA X software (version 10.2.2). The nucleotide sequences of the S glycoproteins, for comparison, were downloaded from the NCBI GenBank database as follows: Conn48725 (FJ899692.
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