HEK293 cells (ATCC# CRL-1573) were cultured in MEM (ATCC) supplemented with 10% FBS (Gibco). Cells were treated for 3 h with 1 μM TL12–186 (Tocris) with the presence of 5 μM MG-132 (Sigma-Aldrich) or treated with only 5 μM MG-132 for 3 h; n = 2 was chosen for each treatment, allowing the investigation of a number of alternative treatment conditions within the same multiplexed experimental (selected results shown). By the end of treatment, HEK293 cells were harvested by Trypsin-0.25% EDTA (Gibco) and washed with cold DPBS. Cell pellets were snap frozen in liquid nitrogen and then stored at −80 °C.
Tl12 186
Manufactured by Bio-Techne
Sourced in United Kingdom
The TL12–186 is a laboratory instrument designed for temperature control applications. It is a thermoelectric module that provides precise temperature regulation for various laboratory processes.
Automatically generated - may contain errors
Lab products found in correlation
Minimum essential medium (mem), by American Type Culture Collection (1 mentions)
Trypsin edta 0.25, by Thermo Fisher Scientific (1 mentions)
Mg132, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nocodazole, by Bio-Techne (1 mentions)
Lenalidomide, by Bio-Techne (1 mentions)
Rabbit anti tubulin antibody, by Abcam (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Tae684, by Selleck Chemicals (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
2 protocols using tl12 186
1
Investigating Proteasome Inhibition in HEK293 Cells
2
Cellular Assay with Chemical Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TL12-186, nocodazole, 2,3-DCPE, and lenalidomide were purchased from Tocris (Bristol, UK), and TAE-684 and MG132 were purchased from Selleckchem (Houston, TX). HEK293 cells were obtained from the American Type Culture Collection and cultured at 37 °C, 5% CO 2 , in DMEM (Gibco, Waltham, MA) containing 10% fetal bovine serum (Seradigm, Radnor, PA). Rabbit anti-CDK2 antibody was purchased from Cell Signaling Technologies (Danvers, MA), and rabbit anti-tubulin antibody was purchased from Abcam (Cambridge, UK).
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