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2 protocols using pure 98 arbutin powder

1

Arbutin-Mediated Cellular Apoptosis Assay

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In this experimental study, Dulbecco’s Modified Eagle
Medium (DMEM) high glucose and RPMI-1640 were
purchased from Biowest (Austria). Fetal bovine serum
(FBS) and penicillin- streptomycin were bought from Gibco
(Germany). Pure (98%) arbutin powder, 2, 4, 6-tripyridyls-
triazine (TPTZ), and 3- [4, 5-dimethylthiazol-2-yl]-2,
5-diphenyltetrazolium (MTT) were purchased from
Sigma-Aldrich (Germany). Annexin V-FITC apoptosis
detection kit was purchased from eBioscience (San
Diego, CA, USA). Tert-butyl hydroperoxide (t-BHP) was
obtained from MERK (Germany) and cDNA synthesis
Kit and YTA qPCR probe MasterMix 2x, were purchased
from Yekta Tajhiz (Iran).
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2

Arbutin Dosage Optimization for Mice

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Pure (98%) arbutin powder (Sigma, Germany) was dissolved in distilled water with concentrations of 50, 100, and 200 mg/kg and injected into mice intraperitoneal 2 hours before irradiation.
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