Atp assay kit

Benz-treated (75, 150, 300 μM for 48 h) and Met-treated SW480 cells were harvested and sonicated in ice three times for 1 min. After centrifugation, supernatants were assayed by ATP assay kit (KeyGen, Nanjing, China) to determine the generation of ATP. The assay was determined using the plate reader (Synergy HT, BioTek, USA) at 636 nm.

Tannic acid was purchased from Shanghai Macklin Biochemical Technology Co., Ltd., (Shanghai, China). MTT was obtained from Solarbio (Beijing, China). The bicinchoninic acid (BCA) protein assay kit, LDH cytotoxicity assay kit, superoxide dismutase (SOD) assay kit, catalase (CAT) assay kit, glutathione peroxidase (GSH-PX) assay kit, lipid peroxidation (MDA) assay kit, and ATP assay kit were purchased from Nanjing KeyGen Biotech. Co. Ltd (Nanjing, China). The Annexin V-FITC apoptosis detection kit and mitochondrial membrane potential assay kit were obtained from Nanjing KeyGen Biotech. Co. Ltd. The ROS content assay kit was purchased from Beyotime Biotech. Co. Ltd. (Nantong, China). Rabbit monoclonal antibody of Bcl-2, Bax, PARP-1, caspase-3, caspase-9, cytochrome c, P53, ATM, P-ATM. CDK, P-CDK, cyclin A2, and β-actin, and goat anti-rabbit antibody of IgG-HRP were obtained from Cell Signaling Technology (Boston, MA, USA). Z-LEHD-FMK was purchased from Abcam, Inc. (Cambridge, UK). The 8-OHdG assay kit was purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).