1 stearoyl 2 linoleoyl sn glycero 3 phosphocholine

1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC), 1,2-diphytanoyl-sn-glycero-3- phosphate (DPhPA), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-snglycero-3-phosphate (DOPA), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1- stearoyl-2-linoleoyl-sn-glycero-3-phosphate (SLPA) and cholesterol in powder form (>99%) were purchased from Avanti Polar Lipids. CaCl₂ (99.999%), poly(vinyl alcohol) (PVA, Mw 146000 - 186000, >99%), bovine serum albumin (BSA, > 99%), glucose, sucrose, and chloroform (>99.8%) were purchased from Sigma-Aldrich. The Ca²⁺ sensitive dye Fluo-4 (Pentapotassium Salt, cell impermeant) was purchased from Thermo-Fisher Scientific. All chemicals were used as received. All aqueous solutions were made with ultra-pure water (H2O, Milli-Q UF plus, Millipore, Inc., electrical resistance of 18.2 MΩ cm). All aqueous solutions were filtered with 0.1 µM Millex filters. The coverslips used in the imaging were precleaned with piranha solution (1:3 - 30% H₂O₂: 95-97% H₂SO₄) and thoroughly rinsed with ultrapure water.

HPLC grade solvents were obtained from VWR International (Mississauga, Ontario, Canada). The standard phospholipids; 1,2-dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (17:0 LPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDHPC), 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PAzPC) and 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PONPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), 1-palmitoyl-2-(5’-keto-6’-octenedioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2(4’-keto-dodec-3’-ene-dioyl)-sn-glycero-3-phosphocholine (KDdiA-PC) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). All other chemicals were purchased from Sigma Aldrich (St. Louis, Missouri, USA).