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1 palmitoyl 2 oleoyl sn glycerol

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1-palmitoyl-2-oleoyl-sn-glycerol is a synthetic lipid compound. It is a glycerol molecule with a palmitic acid chain attached at the sn-1 position and an oleic acid chain attached at the sn-2 position.

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Lab products found in correlation

1 stearoyl 2 docosahexaenoyl sn glycerol, by Avanti Polar Lipids (2 mentions) 1 stearoyl 2 arachidonoyl sn glycerol, by Merck Group (2 mentions) 1 2 dioleoyl sn glycerol, by Avanti Polar Lipids (2 mentions) 1 2 dipalmitoyl sn glycerol, by Avanti Polar Lipids (2 mentions) Phosphatidylserine, by Avanti Polar Lipids (1 mentions) Oleic acid, by Merck Group (1 mentions) Linoleic acid, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Stearic acid, by Merck Group (1 mentions) Precision plus protein dual xtra standard, by Bio-Rad (1 mentions) Simplyblue safestain, by Thermo Fisher Scientific (1 mentions) Arachidonic acid, by Merck Group (1 mentions) Oleoyl l α lysophosphatidic acid sodium salt, by Merck Group (1 mentions) Ans 1 anilinonaphthalene 8 sulfonic acid, by Thermo Fisher Scientific (1 mentions) Cis 5 8 11 14 17 eicosapentaenoic acid, by Merck Group (1 mentions) Cis 4 7 10 13 16 19 docosahexaenoic acid, by Merck Group (1 mentions) Oleoyl coa, by Merck Group (1 mentions) Lc ms grade water, by Merck Group (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) Ammonium formate, by Thermo Fisher Scientific (1 mentions)

6 protocols using 1 palmitoyl 2 oleoyl sn glycerol

1

Lipid-Mediated Protein Kinase C Assay

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1,2-dipalmitoyl-sn-glycerol (16:0/16:0-DG), 1-palmitoyl-2-oleoyl-sn-glycerol (16:0/18:1-DG), 1,2-dioleoyl-sn-glycerol (18:1/18:1-DG), 1-stearoyl-2-docosahexaenoyl-sn-glycerol (18:0/22:6-DG) and phosphatidylserine (PS) were purchased from Avanti Polar Lipids (Alabaster, AL).1-stearoyl-2-arachidonoyl-sn-glycerol (18:0/20:4-DG) and the PKC substrate, the [pGlu4]-Myelin basic protein fragment 4-14 (MBP) was obtained from Sigma-Aldrich (St. Louis, MO). PKC isoforms (α (product number: 01-133), βΙΙ (01-165), γ (01-137), δ (01-135), ε (01-136), η (01-138) and θ (01-140)) were obtained from Carna Biosciences (Kobe, Japan). These PKC isoforms were expressed as N-terminal Glutathione S-transferase-fusion proteins using baculovirus expression system and were highly purified by using glutathione Sepharose chromatography. P81 phosphocellulose squares were obtained from Merck Millipore (Darmstadt, Germany).
Kamiya Y., Mizuno S., Komenoi S., Sakai H, & Sakane F. (2016). Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species. Biochemistry and Biophysics Reports, 7, 361-366.
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2

Antibody-Based Analysis of Human L-FABP

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Antibody against Human L-FABP (H-120), a rabbit polyclonal antibody raised against amino acids 7–126 mapping within an internal region of L-FABP of human origin was purchased from Santa Cruz Biotechnology (Dallas, TX). Antibody against mouse L-FABP was developed in our laboratory as described [102 (link);103 (link)]. Mini-PROTEAN TGX Any kD precast polyacrylamide gels as well as Precision Plus Protein Dual Xtra Standards were purchased from Bio-Rad (Hercules, CA). SimplyBlue SafeStain was obtained from Invitrogen (Carlsbad, CA). ANS (1-anilinonaphthalene-8-sulfonic acid) was purchased from Life Technologies (Grand Island, NY). Stearic acid, palmitic acid, oleic acid, linoleic acid, arachidonic acid (AA), cis-5,8,11,14,17-eicosapentaenoic acid (EPA), cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), Oleoyl CoA, and Oleoyl-L-α-lysophosphatidic acid sodium salt (LPA) were purchased from Sigma (St. Louis, MO). 2-oleoyl glycerol (2-OG), 1-palmitoyl-2-oleoyl-sn-glycerol (PODG), and 1-palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphate monosodium salt (POPA) were obtained from Avanti Polar Lipids (Alabaster, Alabama). All reagents and solvents used were of the highest grade available.
Huang H., McIntosh A.L., Martin G.G., Landrock K.K., Landrock D., Gupta S., Atshaves B.P., Kier A.B, & Schroeder F. (2014). STRUCTURAL AND FUNCTIONAL INTERACTION OF FATTY ACIDS WITH HUMAN LIVER FATTY ACID BINDING PROTEIN (L-FABP) T94A VARIANT. The FEBS journal, 281(9), 2266-2283.
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3

Optimized Lipidomics Workflow

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Methanol (hypergrade), chloroform (LC-MS grade) and water (LC-MS grade) were purchased from Merck (Darmstadt, Germany). 2-propanol and ammonium formate were purchased from Fisher scientific (Waltham, MA, USA). Diacylglycerol reference standards 1,3-di-9Z,12Z-octadecadienolyl)-glycerol, 1,2-dipalmitoyl-sn-glycerol, 1-palmitoyl-2- oleoyl-sn-glycerol, 1-stearoyl-2-arachidonyl-sn-glycerol, 1-stearoyl-2-linoleoyl-sn-glycerol, 1-octadecanoyl-2-hexadecanoyl-sn-glycerol; ceramide reference standards Cer d18:1/14:0, Cer d18:1/16:0, Cer d18:1/18:0, Cer d18:1/18:1 (9Z), Cer d18:1/20:0, Cer d18:2/24:0, Cer d18:1/24:1 (15Z) and internal standards (IS) 1,3(d5)-di-(diheptadecenoyl)-glycerol, Cer d18:1/17:0 were acquired from Avanti polar lipids (Alabaster, AL, USA). Diacylglycerol standards 1,3-dipalmitolein (rac), 1-Palmitin-3-Linolein (rac), 1-Stearin-3-Olein (rac) were purchased from LGC Standards (Wesel, Germany); 1,2-Dimyristoyl-sn-glycerol, 1,2-dioctadecanoyl-sn-glycerol from Cayman Chemical (Ann Arbor, MI, USA) and 1,3-di-(9E-octadecenoyl)-glycerol from Sigma Aldrich (Saint Louis, MO, USA). Protease inhibitor Cocktail (PIC) was purchased from Roche Diagnostics GmbH (Mannheim, Germany).
Preuss C., Jelenik T., Bódis K., Müssig K., Burkart V., Szendroedi J., Roden M, & Markgraf D.F. (2019). A New Targeted Lipidomics Approach Reveals Lipid Droplets in Liver, Muscle and Heart as a Repository for Diacylglycerol and Ceramide Species in Non-Alcoholic Fatty Liver. Cells, 8(3), 277.
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4

Synthesis of Glycerophospholipid Standards

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1-O-1′-(Z)-hexadecenyl-2-oleoyl-sn-glycero-3-phosphocholine, 1-O-1′-(Z)-octadecenyl-2-arachidonoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl-sn-glycerol and 1-stearoyl-2-arachidonoyl-sn-glycerol were purchased from Avanti Polar Lipids. 1-O-hexadecyl-sn-glycero-3-phosphocholine and 1-O-octadecyl-sn-glycero-3-phosphocholine were obtained from Cayman Chemical. Arachidonoyl chloride was purchased from Santa Cruz Biotechnology. Oleoyl chloride, N,N-dimethylglycine (DMG) hydrochloride, 4-(dimethylamino)pyridine, and Bacillus cereus phospholipase C (PLC) were obtained from Sigma Aldrich. Triethylamine was purchased from Fisher Scientific. Solvents for mass spectrometric analysis were purchased from Burdick and Jackson (Muskegon, MI).
Yang K., Jenkins C.M., Dilthey B, & Gross R.W. (2015). Multidimensional Mass Spectrometry-based Shotgun Lipidomics Analysis of Vinyl Ether Diglycerides. Analytical and bioanalytical chemistry, 407(17), 5199-5210.
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5

Characterization of Lipid-Based Reagents

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Cell culture reagents were purchased from Thermo Fisher (Carlsbad, CA, USA), while tissue culture plates and other plastic materials were obtained from Corning Inc. (Corning, NY, USA). Salts and buffers were obtained from Sigma-Aldrich (St. Luis, MI, USA), as well as Thioflavin T (ThT), black Sudan B, Congo red, sodium dodecyl sulfate (SDS), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). l-α-phosphatidylcholine (PC), l-α-phosphatidylserine (PS), l-α-phosphatidyl-ethanolamine (PE), 1-oleoyl-2-hydroxy-sn-glycero-3-phosphate (LPA), 1-palmitoyl-2-oleoyl-sn-glycerol (POPG), and cholesterol were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Antibodies anti-XBP1s and anti-BiP/GRP78 were purchased from Abcam (Cambridge, UK) and anti-β-actin was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-PDI was donated by Dr. Marco A. Ramos Ibarra.
Martínez-Navarro I., Díaz-Molina R., Pulido-Capiz A., Mas-Oliva J., Luna-Reyes I., Rodríguez-Velázquez E., Rivero I.A., Ramos-Ibarra M.A., Alatorre-Meda M, & García-González V. (2020). Lipid Modulation in the Formation of β-Sheet Structures. Implications for De Novo Design of Human Islet Amyloid Polypeptide and the Impact on β-Cell Homeostasis. Biomolecules, 10(9), 1201.
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6

Glycerol Lipid Synthesis Protocol

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1,2-Dioleoyl-sn-glycerol (18:1/18:1-DG), 1-palmitoyl-2-oleoylsn-glycerol (16:0/18:1-DG), 1-stearoyl-2-docosahexaenoylsn-glycerol (18:0/22:6-DG) and PS were purchased from Avanti Polar Lipids (Alabaster, AL, USA). 1-stearoyl-2-arachidonoylsn-glycerol (16:0/20:4-DG) was obtained from Sigma-Aldrich (Tokyo, Japan).
Komenoi S., Takemura F., Sakai H, & Sakane F. (2015). Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol. FEBS letters, 589(11).
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