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Boric acid

Manufactured by Sisco Research Laboratories
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Boric acid is a white, crystalline solid compound that is widely used in various applications. It is the main component of the chemical formula H3BO3 and is known for its diverse industrial and pharmaceutical uses. As a product offered by Sisco Research Laboratories, boric acid serves as a fundamental chemical substance with a core function of being a mild antiseptic and insecticide.

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Lab products found in correlation

D asp, by Merck Group (1 mentions) L asn, by Merck Group (1 mentions) L asp, by Merck Group (1 mentions) Disodium hydrogen phosphate, by Sisco Research Laboratories (1 mentions) Mono sodium hydrogen phosphate, by Sisco Research Laboratories (1 mentions) Glycerol, by Sisco Research Laboratories (1 mentions) Pet28a, by GenScript (1 mentions) Calcein, by Thermo Fisher Scientific (1 mentions) Acrylic acid, by Merck Group (1 mentions) Amicon ultra 0.5 ml centrifugal filter, by Merck Group (1 mentions) Lysotracker blue dnd 22, by Thermo Fisher Scientific (1 mentions) Tris buffer, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) N isopropylacrylamide, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)

2 protocols using boric acid

1

Characterization of l-Asparaginase II

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The commercial l-asparaginase
II, Bionase 5000 IU (Zydus
, India) available as lyophilized powder, was used throughout the
study. Monosodium hydrogen phosphate, disodium hydrogen phosphate,
glycerol (99.5%, molecular biology grade), and boric acid were purchased
from Sisco Research Laboratories Pvt., Ltd. (Mumbai, India). l-Asn, d-Gly, d-Asp, l-Asp, and mushroom
tyrosinase K were purchased from Sigma Aldrich (St. Louis, MO, USA).
The peptide corresponding to the lid loop GGTIAGGGDSATKSNYTAGKVG,
used in the study, was purchased from Creative Biolabs (Shirley, New
York, United States). All buffers were prepared fresh and used within
2 weeks of preparation. The plasmid pET28a encoding the wild-type
sequence of N term 6X-His-tagged l-asparaginase was obtained
from Genscript (USA).
Nag R., Joshi S., Rathore A.S, & Majumder S. (2023). Profiling Enzyme Activity of l-Asparaginase II by NMR-Based Methyl Fingerprinting at Natural Abundance. Journal of the American Chemical Society, 145(19), 10826-10838.
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2

Synthesis and Characterization of Thermo-Responsive Nanomaterials

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N-Isopropylacrylamide (NIPAm),
NIPMAm, rhodamine B, N-(3-aminopropyl)methacrylamide
hydrochloride (APMA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride (EDC), N-hydroxysuccinimide (NHS),
Amicon Ultra 0.5 mL centrifugal filters (MWCO 100 kDa), Triton X-100,
negative control siRNA, and Human Kinase PLK1 siRNA were obtained
from Sigma-Aldrich. Sodium dodecyl sulfate, dimethyl sulfoxide (DMSO),
and acrylic acid were procured from Merck. Ammonium persulfate (APS)
and N,N′-methylenebis(acrylamide)
(BIS) were obtained from Loba Chemie. HeLa cells were obtained from
the National Center for Cell Sciences, Pune, India. Dulbecco’s
modified Eagle’s medium (DMEM, high glucose), Dulbecco’s
phosphate-buffered saline, calcein, Lysotracker blue DND-22, trypsin,
fetal bovine serum (FBS), MTT and SYBR Green II, and Tris buffer were
procured from Thermo Fisher Scientific. PεL (average Mw = 20 000) was obtained from CMS Chemicals
Ltd. (UK) and the FITC Annexin V apoptosis detection kit from BD Biosciences.
Molecular biology-grade agarose (low EEO), glycerol, boric acid, and
disodium ethylenediaminetetraacetic acid (Na2 EDTA) were
procured from Sisco Research Laboratories Pvt. Ltd. (SRL). iScript
cDNA synthesis kit and SsoFast EvaGreen Supermix were procured from
BIORAD. Fluorescein-tagged negative-control siRNA was procured from
Santa Cruz Biotechnology.
Deshpande S., Patil S, & Singh N. (2018). Enhancing Gene-Knockdown Efficiency of Poly(N-isopropylacrylamide) Nanogels. ACS Omega, 3(7), 8042-8049.
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