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2 protocols using aluminum chloride

1

Microbial Compound Extraction and Analysis

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The companies names, cities and countries of used chemicals as followed: methanol (85%), benzene, distilled water and ethanol (70%) from CNW Technologies GmbH (Dusseldorf - Germany). Aluminum chloride from Sangon Biotech (Shanghai- China). Sodium nitrite, sodium bicarbonate and sodium hydroxide from Research Lab Fine Chem. Industries (Maharashtra - India). Dimethyl sulfoxide (DMSO) from Piochem (Giza - Egypt). Gentamycin, when agar from Himedia (Giza - Egypt). Folin-Ciocalteau from Sigma Aldrich (USA). The speacies was authenticated at fungi center, Al-Azhar University (Nasr city - Egypt).
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2

Antioxidant Assays of Natural Compounds

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Ascorbic acid (ASA), catechin, chloranil, 2,6-dichloroindophenol sodium salt hydrate (DIP), 2,2′-azobis-amidinopropane (ABAP), Trolox, Folin-Ciocalteu reagent, and gallic acid (GA) were purchased from Sigma (St. Louis, MO, USA). Metaphosphoric acid, acetone, alcohol, hydrochloric acid (HCl), ethyl acetate, sodium carbonate (Na2CO3), tetrahydrofuran (THF), vanillin, sodium borohydride (NaBH4), methanol, and fluorescein disodium salt were purchased from Aladdin (Shanghai, China). Acetic acid, sodium hydroxide (NaOH), potassium hydroxide, potassium phosphate dibasic, potassium phosphate monobasic, aluminum chloride, and sodium bicarbonate (NaHCO3) were acquired from Sangon Company (Shanghai, China).
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