The human esophageal epithelial cell line (Het-1a) and the EC cell lines EC9706, Eca109, EC1, KYSE30 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences. Esophageal cancer cell lines were maintained in RPMI 1640 medium with 10% fetal bovine serum (FBS; Gibco) and were incubated at 37 °5% CO2. Het-1a cell line was maintained in Dulbecco’s Modified Eagle medium with high glucose. The small interfering RNA, microRNA (miRNA) mimics, and inhibitors were purchased from Shanghai Genepharma Co, Ltd and were transfected into cells with the BTX ECM 2001 square wave electroporator according to the BTX Molecular Delivery Systems protocol.
Eca109
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China, United States
The Eca109 is a laboratory cell culture equipment used for the cultivation and maintenance of the Eca109 cell line. It provides a controlled environment for the growth and analysis of this specific cell type.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (36 mentions)
Kyse150, by National Collection of Authenticated Cell Cultures (34 mentions)
Ec9706, by National Collection of Authenticated Cell Cultures (23 mentions)
Het 1a, by National Collection of Authenticated Cell Cultures (20 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (18 mentions)
Rpmi 1640, by Thermo Fisher Scientific (11 mentions)
Kyse450, by National Collection of Authenticated Cell Cultures (9 mentions)
Kyse30, by National Collection of Authenticated Cell Cultures (8 mentions)
Eca 109, by Thermo Fisher Scientific (7 mentions)
Kyse140, by National Collection of Authenticated Cell Cultures (7 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
Het 1a, by American Type Culture Collection (6 mentions)
Kyse510, by National Collection of Authenticated Cell Cultures (6 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (6 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions)
Te 13, by National Collection of Authenticated Cell Cultures (4 mentions)
Eca9706, by National Collection of Authenticated Cell Cultures (4 mentions)
Kyse410, by National Collection of Authenticated Cell Cultures (4 mentions)
93 protocols using eca109
1
Esophageal Cancer Cell Line Transfection
2
ESCC Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3
Culturing Human ESCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human ESCC cell lines Eca109 and EC1 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), and cultured in media at 37°C with 5% CO2. All media consisted of Dulbecco’s Modified Eagle’s Medium (DMEM, BasalMedia, Shanghai, China), 10% fetal bovine serum (FBS, Biochrom AG, Berlin, Germany), and 1% penicillin-streptomycin solution (BasalMedia, Shanghai, China).
4
Culturing Esophageal and EC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human esophageal cell line, HET-1A, and EC cell lines Eca109, EC-1, EC9706, KYSE30 and KYSE150 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Gibco, Australia), 100 U/mL penicillin, and 50 μg/mL streptomycin, and were kept in incubators with humidified atmospheres of 5% CO2 and 95% air at 37°C.
5
Esophageal Cancer Cell Line Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patients and cell lines. Patients analyzed in this study were diagnosed with EC during the period 1999-2014 at Anshan Cancer Hospital (Anshan, Liaoning, China). None of the patients had been administered chemotherapy or radiotherapy prior to surgery. In total, 83 formalin-fixed, paraffin-embedded specimens were obtained from resected tumors and 11 adjacent normal specimens. The patient age ranged from 31 to 78 years with a mean age of 59.8±10.83 years. Tumors were graded according to the WHO grading system and staged according to the modified Astler-Coller system (MAC).
The human esophageal squamous cancer cell lines (Eca-109 and TE-1) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Eca-109 was cultured in RPMI-1640, supplemented with 10% fetal bovine serum (FBS) (both from Gibco-Life Technologies, Carlsbad, CA, USA) and 100 µg/ml each of penicillin and streptomycin (Invitrogen-Life Technologies, Carlsbad, CA, USA). The TE-1 was cultured in complete medium of highglucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% (FBS) (Gibco-Life Technologies) and 100 µg/ml each of penicillin and streptomycin (Invitrogen-Life Technologies). The cells were maintained in a humidified 5% CO 2 incubator at 37˚C.
The human esophageal squamous cancer cell lines (Eca-109 and TE-1) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Eca-109 was cultured in RPMI-1640, supplemented with 10% fetal bovine serum (FBS) (both from Gibco-Life Technologies, Carlsbad, CA, USA) and 100 µg/ml each of penicillin and streptomycin (Invitrogen-Life Technologies, Carlsbad, CA, USA). The TE-1 was cultured in complete medium of highglucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% (FBS) (Gibco-Life Technologies) and 100 µg/ml each of penicillin and streptomycin (Invitrogen-Life Technologies). The cells were maintained in a humidified 5% CO 2 incubator at 37˚C.
6
Eca-109 and TE-1 Cell Culture and Endostar Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eca-109 and TE-1 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (both from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and were incubated at 37°C in a humidified incubator with 5% CO2. Endostar was aliquoted to achieve desired working volumes of 20 µl and stored at 4°C. The drug was further diluted with DMEM to 25, 50, 100 and 200 µg/ml prior to use.
7
Esophageal Cancer Cell Lines and Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human EC cell lines Eca-109 (primary tumor-derived), TE-1 (primary tumor-derived), and KYSE-150 (primary tumor-derived) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The normal human esophageal epithelial cell line Het-1A was obtained from American Type Culture Collection (Manassas, VA, U.S.A.) and used as a control cell. Cells were cultivated in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, U.S.A.) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific), streptomycin (100 U/ml) (Thermo Fisher Scientific), and penicillin (100 U/ml) (Thermo Fisher Scientific) at 37°C with an atmosphere of 5% CO2. MiR-1294 mimic, miR-1294 inhibitor, specific siRNA target to hsa_circ_0004370 (si- circ_0004370: GCGUCUCCGUACAGAUGACCATT, targeting covalent closed junction) or LASP1 (si-LASP1: GAAUCAAGAAGACCCAGGATT, targeting 458-476), and corresponding controls (si-NC: UUCUCCGAACGUGUCACGUTT) were designed and provided by RiboBio (Guangzhou, China). Cells were cultured till 60–70% confluence and then the transfection was performed using Lipofectamine 2000 (Invitrogen, CA, U.S.A.) as described in manual.
8
Culturing ESCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ESCC cell lines TE-9 and Eca-109 were purchased from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The cell lines were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin, and cultured in a humidified atmosphere of 37°C with 5% CO2.
9
ESCC Cell Line Culturing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The three human ESCC cell lines (KYSE150, ECA-109 and EC9706) and normal epithelial cell line (HET-1A) were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All cell lines were maintained in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a humidified chamber with 5% CO2.
10
Culturing Human Esophageal Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ESCC cell lines KYSE30, KYSE150, KYSE450, KYSE510 and Eca109 and human normal esophageal epithelial cell line Het-1A were obtained from the Type Culture Collection of the Chinese Academy of Sciences and cultured in RPMI-1640 medium supplemented with fetal bovine serum (FBS) (10%) at 37°C in 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!