CD3 (reference range (700–2100) × 109/L), CD4 (reference range (300–1400) × 109/L), CD8 (reference range (200–900) × 109/L), CD19 (reference range (100–900) × 109/L), and CD56 (reference range (90–600) × 109/L) markers were done by the Department of Immunology, Central Manchester University Hospitals NHS Foundation Trust (CMFT) (Clinical Science Building 3, Manchester Royal Infirmary, Oxford Road, Manchester, UK). This facility is accredited (IS0 15189:2012) for CD enumeration using FC500 MPL flow cytometer equipment (Beckman Coulter Ltd., Buckinghamshire, UK). The dates of sampling were recorded. Absolute T- and B-lymphocytes and NK-cell CD counts were recorded; the laboratory does not provide percentage CDs, the reference ranges given are derived from healthy Europeans. Changes in reference ranges from 16th November 2015 were noted. The current reference ranges were used for statistical analyses, even for results produced before 16 November 2015. The unit of all CD biomarkers used in this study is (×109/L).
Fc500 mpl flow cytometer equipment
Manufactured by Beckman Coulter
Sourced in United Kingdom
The FC500 MPL flow cytometer is a laboratory instrument designed for the analysis and enumeration of cells. It utilizes flow cytometry technology to detect and measure various characteristics of cells, including size, granularity, and the presence of specific markers on the cell surface. The FC500 MPL is capable of analyzing a wide range of cell types and is commonly used in fields such as immunology, hematology, and cell biology research.
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Lab products found in correlation
2 protocols using fc500 mpl flow cytometer equipment
1
Lymphocyte Subset Analysis by Flow Cytometry
2
Comprehensive Immunological Biomarker Profiling in Aspergillosis
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Patient data was obtained from the National Aspergillosis Centre database, encompassing a period of three years prior to sample collection. We collected all available results for the following immunological biomarkers: mannose binding lectin (MBL), IgG, IgA, IgM, pneumococcal antibody titre (Pn Ab), Haemophilus antibody titre (Hm Ab), gamma interferon (IFNg) production, interleukin 12 (IL12) production, interleukin 17 (IL17) production, CD3, CD4, CD8, CD19, and CD56 (Fig. 1 ). Cytokine production assays were only ordered in patients failing antifungal therapy or those with dual mycobacterial and Aspergillus infections.
CD3 (reference range (700–2100) × 109/L), CD4 (reference range (300–1400) × 109/L), CD8 (reference range (200–900) × 109/L), CD19 (reference range (100–900) × 109/L), and CD56 (reference range (90–600) × 109/L) markers were done by the Manchester University NHS Foundation Trust, Department of Clinical Immunology. This facility is accredited (IS0 15189:2012) for CD enumeration using FC500 MPL flow cytometer equipment (Beckman Coulter Ltd., Buckinghamshire, UK).
CD3 (reference range (700–2100) × 109/L), CD4 (reference range (300–1400) × 109/L), CD8 (reference range (200–900) × 109/L), CD19 (reference range (100–900) × 109/L), and CD56 (reference range (90–600) × 109/L) markers were done by the Manchester University NHS Foundation Trust, Department of Clinical Immunology. This facility is accredited (IS0 15189:2012) for CD enumeration using FC500 MPL flow cytometer equipment (Beckman Coulter Ltd., Buckinghamshire, UK).
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