Cultured cells were trypsinized, washed, and resuspended in Hank’s Balanced Salt Solution (Lonza, Basel, Switzerland) supplemented with 1% HEPES and 2% FBS. Cells were then incubated with antibodies on ice for 30 min. Labeled cells were analyzed by FACS using a FACSCalibur (BD Biosciences, San Jose, CA). The antibodies used were: a FITC-conjugated anti-EpCAM monoclonal antibody (Clone Ber-EP4; DAKO, Carpinteria, CA); an APC-conjugated anti-CD326 (EpCAM) antibody (Miltenyi Biotec K.K., Tokyo, Japan); an APC-conjugated anti-CD90 monoclonal antibody (Clone 5E10; eBioscience, San Diego, CA); an APC-conjugated anti-CD133/2 antibody (Clone 293C3; Miltenyi Biotec K.K.); an APC-conjugated anti-CD44 mouse monoclonal antibody (eBioscience); an APC-conjugated anti-CD13 antibody (eBioscience); and a PE-conjugated anti-CD24 antibody (Miltenyi Biotec K.K.).
Apc conjugated anti cd326 epcam antibody
Manufactured by Miltenyi Biotec
Sourced in Canada
The APC-conjugated anti-CD326 (EpCAM) antibody is a laboratory reagent used for the detection and analysis of cells expressing the EpCAM antigen. It is conjugated with the fluorescent dye Allophycocyanin (APC) for easy identification and quantification of target cells by flow cytometry.
Automatically generated - may contain errors
2 protocols using apc conjugated anti cd326 epcam antibody
1
Multiparametric Flow Cytometry of Stem Cell Markers
2
Immunophenotyping of Cultured Cells
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Cultured cells were trypsinized, washed, and resuspended in Hank's balanced salt solution (Lonza, Basel, Switzerland) supplemented with 1% HEPES and 2% FBS. The cells were then incubated with antibodies on ice for 30 minutes. Labeled cells were analyzed by FACS using a FACSCalibur (BD Biosciences, San Jose, CA). The following antibodies were used: FITC-conjugated anti-EpCAM monoclonal antibody Clone Ber-EP4 (DAKO, Carpinteria, CA), APC-conjugated anti-CD326 (EpCAM) antibody (Miltenyi Biotec K.K., Tokyo, Japan), FITC-conjugated anti-CD90 monoclonal antibody Clone 5E10 (STEMCELL Technologies, Seattle, WA), APC-conjugated anti-CD133/2 antibody Clone 293C3 (Miltenyi Biotec K.K.), and APC-conjugated anti-CD105 mouse monoclonal antibody (BD Biosciences).
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