For surgery, animals were anesthetized with a mixture of fentanyl (0.05 mg/kg bodyweight; Sintenyl; Sintetica), midazolam (5 mg/kg bodyweight; Dormicum, Roche), and medetomidine (0.5 mg/kg bodyweight; Domitor; Orion Pharma), administered intraperitoneally. Anesthesia was maintained with midazolam (5 mg/kg bodyweight), injected subcutaneously 50 min after induction. To prevent hypoxemia, a face mask provided 300 ml/min of 100% oxygen. During two-photon imaging mice were anesthetized with 1.2% isoflurane (Attane TM ; Piramal Healthcare, India) and supplied with 300 ml/min of 100% oxygen. Core temperature was kept constant at 37 °C using a homeothermic heating blanket system (Harvard Apparatus) during all surgical and experimental procedures. The animal`s head was fixed in a stereotaxic frame (Kopf Instruments) and the eyes were kept moist with ointment (vitamin A eye cream; Bausch & Lomb).
Homeothermic heating blanket system
Manufactured by Harvard Apparatus
The Homeothermic heating blanket system is a laboratory device designed to maintain a constant temperature for various applications. It provides a reliable and controlled heating environment for experimental samples or subjects. The system includes a heating blanket and associated control components to regulate the temperature as required for the specific research or experimental needs.
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Lab products found in correlation
2 protocols using homeothermic heating blanket system
1
Anesthesia and Imaging Protocol for Mice
2
Anesthesia and Monitoring for In Vivo Imaging
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For surgery, animals were anesthetized with a mixture of fentanyl (0.05 mg/kg bodyweight; Sintenyl; Sintetica), midazolam (5 mg/kg bodyweight; Dormicum, Roche), and medetomidine (0.5 mg/kg bodyweight; Domitor; Orion Pharma), administered intraperitoneally. Anesthesia was maintained with midazolam (5 mg/kg bodyweight), injected subcutaneously 50 min after induction. To prevent hypoxemia, a face mask provided 300 ml/min of 100% oxygen. During two-photon imaging mice were anesthetized with 1.2% isoflurane (Attane; Piramal Healthcare, India) and supplied with 300 ml/min of 100% oxygen. Core temperature was kept constant at 37°C using a homeothermic heating blanket system (Harvard Apparatus) during all surgical and experimental procedures. The animal’s head was fixed in a stereotaxic frame (Kopf Instruments) and the eyes were kept moist with ointment (vitamin A eye cream; Bausch & Lomb).
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