Leibovitz s l15 medium

Human breast cancer MDA-MB-231 cells were purchased from the Institute of Basic Medical Science, Chinese Academy of Medical Science (Beijing, China). Cells were grown in Leibovitz's L15 medium (Macgene, Beijing, China) supplemented with 10% fetal bovine serum (FBS, Gibco, Billings, USA) in a 37°C humidified incubator. Female BALB/c nude mice (initial weight of 16–18 g) were obtained from the Peking University Experimental Animal Center (Beijing, China). All animal experimental procedures were performed with the approval of the Institutional Authority for Laboratory Animal Care of Peking University according to the guidelines.

Human breast cancer cell lines (MDA-MB-435S, MDA-MB-231, and MCF-7) were purchased from the Institute of Basic Medical Science, Chinese Academy of Medical Science (Beijing, China) and used below passage 10. MDA-MB-435S and MDA-MB-231 cells were grown in Leibovitz’s L15 medium (Macgene Biotech Co., Ltd., Beijing, China) supplemented with 10% fetal bovine serum (FBS, PAN, Adenbach, Germany) in a 37 °C humidified incubator. MCF-7 cells were cultured in RPMI 1640 (Macgene Biotech Co., Ltd., Beijing, China) supplemented with 10% FBS in a humidified incubator at 37 °C under 5% CO₂.