Primary antibodies used were from the following sources: rabbit (
Rb) anti-Myc (Cell Signaling, 2278), mouse (
Ms) anti-Myc (Cell Signaling, 9B11),
Rb anti-actin (Sigma, A2066),
Ms anti-FLAG (Sigma F1804),
Rb anti-14-3-3 (Santa Cruz, sc-629),
Ms anti -Spir-1 (Santa Cruz, sc-517039), Ms anti-Spir-1 (Abcam, ab57463),
Rb anti-DDX3 (Cell Signaling, 2635), Rb anti-IKKβ (Cell Signaling, 2684),
Rb anti-HA (Sigma, H6908), Ms anti-α-tubulin (Millipore, 05–829),
Ms anti-GAPDH (Sigma, G8795),
Rb anti-IRF3 (Cell Signaling, 4962),
Rb anti-IRF3 (Santa Cruz, SC-9082), Ms anti-COPε (Santa Cruz, sc-133194),
Rb anti-phospho-IRF3 Ser396 (Cell Signaling, 4947S) and Rb polyclonal anti-C6 [53 (
link)]. For dilutions used for the primary antibodies, see
S3 Table. Secondary antibodies used (1:10,000 dilution) were
IRDye 680RD-conjugated goat anti-rabbit IgG or anti-mouse IgG and
IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG (LI-COR).
Reagents used in this study were:
anti-c-Myc agarose from Santa Cruz Biotechnology, and monoclonal anti-HA-agarose, clone HA-7,
ANTI-FLAG M2 affinity gel and
Poly-D-lysine hydrobromide (all from Sigma Aldrich).
Human IFNα,
human TNF-α and
mouse IL-1β were from Peprotech,
HMW poly(I:C) and
puromycin were from InvivoGen, and doxycycline was from Melford.
Torres A.A., Macilwee S.L., Rashid A., Cox S.E., Albarnaz J.D., Bonjardim C.A, & Smith G.L. (2022). The actin nucleator Spir-1 is a virus restriction factor that promotes innate immune signalling. PLoS Pathogens, 18(2), e1010277.