Faecalibacterium sp. isolates and the reference strain DSM 17677 were inoculated in 25 ml of VTR2RF broth and incubated at 37°C for 48 h under anaerobic conditions. The culture was centrifuged at 4000×g for 10 min and the supernatant was collected. The concentration of acetate, butyrate, propionate and isobutyrate in the VTR2RF media before inoculation and in the supernatant of the culture was measured by gas chromatography. Samples were injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich, St. Louis, MO) and the analysis was performed according to the manufacturer's protocol [26] at the Dairy One Cooperative, Ithaca, NY. The procedure was repeated, at least, 3 times for each sample.
Supelco packed column
Manufactured by Merck Group
Supelco packed column is a laboratory chromatography column designed for analytical separation and purification of chemical compounds. It is filled with a solid stationary phase material packed inside the column. The column can be used for various chromatographic techniques, such as gas chromatography or liquid chromatography, to separate and analyze complex mixtures.
Automatically generated - may contain errors
4 protocols using supelco packed column
1
Anaerobic Cultivation and Metabolite Analysis of Faecalibacterium sp.
Check if the same lab product or an alternative is used in the 5 most similar protocols
Faecalibacterium sp. isolates and the reference strain DSM 17677 were inoculated in 25 ml of VTR2RF broth and incubated at 37°C for 48 h under anaerobic conditions. The culture was centrifuged at 4000×g for 10 min and the supernatant was collected. The concentration of acetate, butyrate, propionate and isobutyrate in the VTR2RF media before inoculation and in the supernatant of the culture was measured by gas chromatography. Samples were injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich, St. Louis, MO) and the analysis was performed according to the manufacturer's protocol [26] at the Dairy One Cooperative, Ithaca, NY. The procedure was repeated, at least, 3 times for each sample.
+ Expand
Faecalibacterium sp. isolates and the reference strain DSM 17677 were inoculated in 25 ml of VTR2RF broth and incubated at 37°C for 48 h under anaerobic conditions. The culture was centrifuged at 4000×g for 10 min and the supernatant was collected. The concentration of acetate, butyrate, propionate and isobutyrate in the VTR2RF media before inoculation and in the supernatant of the culture was measured by gas chromatography. Samples were injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich, St. Louis, MO) and the analysis was performed according to the manufacturer's protocol [26] at the Dairy One Cooperative, Ithaca, NY. The procedure was repeated, at least, 3 times for each sample.
2
Evaluating Bacterial Metabolite Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 34
Bacterial isolates or evolved laboratory strains are inoculated in 25 ml of Versa TREK REDOX 2 broth (Trek Diagnostic Systems) supplemented with 30% sterile-filtered cow rumen fluid and prebiotic of interest incubated under anaerobic conditions for 2 days at 37° C. Following incubation, cultures are centrifuged at 6000 rpm for 10 min. Supernatants, uninoculated medium, or standards of short chain fatty acids (e.g., acetate, propionate, butyrate (Sigma-Aldrich) are injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich) according to the manufacturer's protocol (Dairy One Cooperative) (Foditsch C et al., 2014. Isolation and Characterization of Faecalibacterium prausnitzii from Calves and Piglets. PLoS One. 9(12): e116455). Bacterial isolates are selected with maximal short chain fatty acid (e.g., butyrate) production. Similar kind of analysis is done to access impact of prebiotics on other bacterial metabolite production such as but not limited to secondary bile acids.
3
Anaerobic Screening of Bacterial Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 34
Bacterial isolates or evolved laboratory strains are inoculated in 25 ml of Versa TREK REDOX 2 broth (Trek Diagnostic Systems) supplemented with 30% sterile-filtered cow rumen fluid and prebiotic of interest incubated under anaerobic conditions for 2 days at 37° C. Following incubation, cultures are centrifuged at 6000 rpm for 10 min. Supernatants, uninoculated medium, or standards of short chain fatty acids (e.g., acetate, propionate, butyrate (Sigma-Aldrich) are injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich) according to the manufacturer's protocol (Dairy One Cooperative) (Foditsch C et al., 2014. Isolation and Characterization of Faecalibacterium prausnitzii from Calves and Piglets. PLoS One. 9(12): e116455). Bacterial isolates are selected with maximal short chain fatty acid (e.g., butyrate) production. Similar kind of analysis is done to access impact of prebiotics on other bacterial metabolite production such as but not limited to secondary bile acids.
4
Evaluating Bacterial Metabolite Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 34
Bacterial isolates or evolved laboratory strains are inoculated in 25 ml of Versa TREK REDOX 2 broth (Trek Diagnostic Systems) supplemented with 30% sterile-filtered cow rumen fluid and prebiotic of interest incubated under anaerobic conditions for 2 days at 37° C. Following incubation, cultures are centrifuged at 6000 rpm for 10 min. Supernatants, uninoculated medium, or standards of short chain fatty acids (e.g., acetate, propionate, butyrate (Sigma-Aldrich) are injected into a Perkin Elmer Autosystem XL Gas Chromatograph containing a Supelco packed column (Sigma-Aldrich) according to the manufacturer's protocol (Dairy One Cooperative) (Foditsch C et al., 2014. Isolation and Characterization of Faecalibacterium prausnitzii from Calves and Piglets. PLoS One. 9(12): e116455). Bacterial isolates are selected with maximal short chain fatty acid (e.g., butyrate) production. Similar kind of analysis is done to access impact of prebiotics on other bacterial metabolite production such as but not limited to secondary bile acids.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!