CAPE procured from Sigma-Aldrich was dissolved in sterile dimethyl sulfoxide (DMSO) diluted with isotonic saline (1:5). STZ was procured from Sisco Research Laboratories Pvt. Ltd., Mumbai. Dihydrogen orthophosphate, 5,5’-dithiobis (2-nitrobenzoic acid) (DTNB) (Himedia Laboratories, Mumbai); thiobarbituric acid, trichloroacetic acid, sodium dodecyl sulphate (Loba Chemie, Mumbai); and TNF-α ELISA kit (Krishgen, Mumbai) were used. Artificial cerebrospinal fluid (aCSF) was prepared as follows: (in mmol/L) 147 mM NaCl, 2.9 mM KCl, 1.6 mM MgCl2, 1.7 mM CaCl2, 2.2 mM dextrose were dissolved in 10 mL of water for injection.[9 (link)] All the drug solutions were freshly prepared before injections.
Sodium dodecyl sulphate (sds)
Manufactured by Loba Chemie
Sourced in Egypt
Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant and detergent that is commonly used in various laboratory applications. It is a white to yellowish crystalline powder that is soluble in water and other polar solvents. SDS is known for its ability to denature proteins and disrupt cell membranes, making it a valuable tool in biochemical and molecular biology research.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Sisco Research Laboratories (1 mentions)
Thiobarbituric acid, by Loba Chemie (1 mentions)
Trichloroacetic acid, by Loba Chemie (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Loba Chemie (1 mentions)
Hydrochloric acid (hcl), by Loba Chemie (1 mentions)
Peg 400, by Loba Chemie (1 mentions)
Peg 6000, by Loba Chemie (1 mentions)
Dichloromethane, by Loba Chemie (1 mentions)
Acetone, by Loba Chemie (1 mentions)
Span 80, by Loba Chemie (1 mentions)
Tween 80, by Loba Chemie (1 mentions)
Microcrystalline cellulose, by Loba Chemie (1 mentions)
3 protocols using sodium dodecyl sulphate (sds)
1
CAPE and STZ-Induced Neuroinflammation
2
Analytical Method for Antidiabetic Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pharco Pharmaceuticals, Co. (Alexandria, Egypt) kindly offered MET and LIN, while EMP was supplied by Pharaonia pharmaceuticals (Alexandria, Egypt). HPLC grade methanol (Fisher Scientific UK Limited, Loughborough, Leicestershire, UK), Tris buffer Extra pure and sodium phosphate dibasic (Loba Chemie, Mumbai, India), sodium hydroxide, hydrochloric acid, sodium dodecyl sulphate (El-Nasr chemical Co., Giza, Egypt) and deionized water were used.
Empagliform® tablets labeled to contain 12.5 mg EMP/ 500 mg MET, Gliptalina® tablets labeled to contain 2.5 mg LIN/ 500 mg MET and Glyxambi® tablets labeled to contain 10 mg EMP/ 5 mg LIN were purchased from the Egyptian market. Trijardy XR® tablets labeled to contain 25 mg EMP/ 5 mg LIN/ 1000 mg MET, due to the unavailability in the local market, laboratory-prepared tablets having the same drugs’ amount were prepared. This was done by using the individual dosage forms of each drug alone Trajenta®, Empaglimax® and Cidophage® labelled to contain 5 mg, 25 mg and 1000 mg per tablet, respectively.
Empagliform® tablets labeled to contain 12.5 mg EMP/ 500 mg MET, Gliptalina® tablets labeled to contain 2.5 mg LIN/ 500 mg MET and Glyxambi® tablets labeled to contain 10 mg EMP/ 5 mg LIN were purchased from the Egyptian market. Trijardy XR® tablets labeled to contain 25 mg EMP/ 5 mg LIN/ 1000 mg MET, due to the unavailability in the local market, laboratory-prepared tablets having the same drugs’ amount were prepared. This was done by using the individual dosage forms of each drug alone Trajenta®, Empaglimax® and Cidophage® labelled to contain 5 mg, 25 mg and 1000 mg per tablet, respectively.
3
Formulation and Evaluation of Glimepiride
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glimepiride was kindly provided by Swiss Garnier Life Sciences (Mehatpur, Una, Himachal Pradesh, India) as a gift sample. Acetone, dichloromethane, polyethylene glycol (PEG400, PEG6000), Span 80, Tween 80, polyvinyl pyrolidone (PVP K30), microcrystalline cellulose and sodium dodecyl sulphate were purchased from Loba Chemie Pvt. Ltd.
(Mumbai, India). All other chemicals and reagents were of analytical grade.
(Mumbai, India). All other chemicals and reagents were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!