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Valdecoxib

Manufactured by Merck Group
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Valdecoxib is a pharmaceutical product developed by Merck Group. It is a selective COX-2 inhibitor, a type of non-steroidal anti-inflammatory drug (NSAID). The core function of Valdecoxib is to provide anti-inflammatory, analgesic, and antipyretic effects by selectively inhibiting the COX-2 enzyme.

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Lab products found in correlation

Celecoxib, by Merck Group (5 mentions) Etoricoxib, by Merck Group (2 mentions) Piroxicam, by Merck Group (2 mentions) Rofecoxib, by Merck Group (2 mentions) Celltiter glo luminescent cell viability assay atp, by Promega (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) Dantrolene, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Cyclopiazonic acid, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Xestospongin c, by Merck Group (1 mentions) Monoclonal flag clone m2, by Merck Group (1 mentions) A23187, by Merck Group (1 mentions) Glutamate, by Merck Group (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions) 2 5 dimethyl celecoxib, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions)

5 protocols using valdecoxib

1

Antibody and Chemical Reagents in Cell Studies

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The antibodies used were monoclonal GFP (Roche Applied Science, Indianapolis, IN), monoclonal actin (clone C4; Abcam, Cambridge, MA), polyclonal actin (Sigma-Aldrich), polyclonal Gaussia luciferase (New England Biolabs), polyclonal BiP (Cell Signaling, Danvers, MA), monoclonal FLAG (clone M2; Sigma-Aldrich), and monoclonal Myc (clone 4A6; Millipore). Chemicals used (with diluent in parentheses) were thapsigargin (dimethyl sulfoxide [DMSO] in in vitro studies, ethanol in in vivo studies; Sigma-Aldrich), cycloheximide (DMSO; Sigma-Aldrich), brefeldin A (ethanol; Sigma-Aldrich), dithiothreitol (water; Sigma-Aldrich), tunicamycin (DMSO; Sigma-Aldrich), cyclopiazonic acid (DMSO; Sigma-Aldrich), A23187 (DMSO; Sigma-Aldrich), TPEN (DMSO; Sigma-Aldrich), dantrolene (DMSO; Sigma-Aldrich), xestospongin C (DMSO; Sigma-Aldrich), caffeine (water; Sigma-Aldrich), glutamate (1 N HCl; Sigma-Aldrich), celecoxib (DMSO; Sigma-Aldrich), valdecoxib (DMSO; Sigma-Aldrich), and 2,5-dimethyl-celecoxib (DMSO; Sigma-Aldrich). Vehicle controls at concentrations equivalent to treatments were used in all experiments. Cell viability assays were performed with the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) or the CellTiter-Glo Luminescent Cell Viability Assay (ATP) following the manufacturer's instructions (Promega, Madison, WI).
Henderson M.J., Wires E.S., Trychta K.A., Richie C.T, & Harvey B.K. (2014). SERCaMP: a carboxy-terminal protein modification that enables monitoring of ER calcium homeostasis. Molecular Biology of the Cell, 25(18), 2828-2839.
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2

Extraction and Characterization of NSAIDs

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The compounds: piroxicam, tenoxicam, meloxicam, isoxicam, celecoxib, etoricoxib, rofecoxib, valdecoxib and firocoxib were obtained from Sigma-Aldrich. Cimicoxib and robenacoxib were extracted from Cimalgex tablets (Vetoquinol S.A., Lure, France) and Onsior tablets (Novartis Sante Animale S.A.S., Basingstoke, UK), respectively. Analytical grade methanol, acetone and water were purchased from Merck (Darmstadt, Germany).
Starek M., Plenis A., Zagrobelna M, & Dąbrowska M. (2021). Assessment of Lipophilicity Descriptors of Selected NSAIDs Obtained at Different TLC Stationary Phases. Pharmaceutics, 13(4), 440.
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3

Quantitative Analysis of COX-2 Inhibitors

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Parecoxib, valdecoxib, and celecoxib, all of which with purity > 98.0% were obtained from Sigma-Aldrich (St. Louis, MO, USA). LC-MS grade acetonitrile and formic acid (98% purity) were procured from Merck (Darmstadt, Germany) and Sigma-Aldrich (Munich, Germany), respectively. Other organic solvents born with HPLC grade were purchased from Merck (Darmstadt, Germany). It is worth mentioning that the rest of the reagents employed throughout this study were of analytical pure without further purification, include the ultra-pure water, which was yielded by a Millipore Milli-Q purification system (Billerica, MA, USA).
Chen M., Sun W., Wang Z., Huang C., Hu G., Chen Y, & Wang L. (2020). Determination of parecoxib and valdecoxib in rat plasma by UPLC-MS/MS and its application to pharmacokinetics studies. BMC Pharmacology & Toxicology, 21, 27.
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4

Isolation and Culture of Tissue Samples

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As described previously,21 (link) harvested tissue samples were immediately placed in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum, 200 μg/mL ampicillin, and 200 μg/mL streptomycin. Tissues were cut into small pieces and transferred into a sterile T-25 flask with dispase solution (Gibco®, Thermo Fisher Scientific, Waltham, MA, USA), and then incubated overnight at 37°C. After centrifu-gation for 5 minutes at 1,400× g, the samples were transferred to culture dishes in a 1:1 (v/v) mixture of Dulbecco’s Modified Eagle’s Medium/Ham’s F12. The cultures were incubated at 37°C in a humidified atmosphere with 5% CO2 for approximately 2 weeks. The selective COX-2 inhibitors celecoxib and valdecoxib (Sigma-Aldrich Co, St Louis, MO, USA) were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich Co) and then added into the culture medium for experiments as previously reported.22 (link),23 (link)
Wei G., Chen X., Wang G., Jia P., Xu Q., Ping G., Wang K, & Li X. (2015). Inhibition of cyclooxygenase-2 prevents intra-abdominal adhesions by decreasing activity of peritoneal fibroblasts. Drug Design, Development and Therapy, 9, 3083-3098.
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5

Comprehensive Anti-Inflammatory Compound Database

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Aceclofenac, diclofenac, DMSO, Hexafluoroisopropanol (HFIP), ibuprofen, ketoprofen, meloxicam, naproxen, nimesulide, phenylbutazone, resveratrol, resazurin, silibinin, sulindac, tenoxicam and thioflavin-T (ThT) were obtained from Alfa Aesar (Ward Hill, MA). Celecoxib, etoricoxib, indometacin, ketorolac, flurbiprofen, niflumic acid, oxaprozin, piroxicam, rofecoxib and valdecoxib were purchased from Sigma-Aldrich (St. Louis, MO).
Fortin J.S, & Benoit-Biancamano M.O. (2016). Inhibition of islet amyloid polypeptide aggregation and associated cytotoxicity by nonsteroidal anti-inflammatory drugs. Canadian journal of physiology and pharmacology, 94(1).
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