A 11004

Primary antibodies used were: rabbit anti-dtTomato dsRed (1:1000; Clontech, 632496), anti-olig2 (1:500, Millipore, AB9610) and anti-cleaved caspase-3 (1:1000: Cell Signalling Technology, 9661); mouse anti-BrdU (IgG₁; 1:200, Sigma-Aldrich, B2531), anti-GFAP (IgG₁, 1:1000, Millipore, MAB360), anti-NeuN (IgG₁, 1:1500, Millipore, MAB377), anti-PCNA (IgG_2a; 1:150, Millipore, MAB424R) and anti-S100β (1:200, Abcam, Ab4066). Secondary antibodies of the relevant species and subclass, either coupled to biotin-Streptavidin Cy2 or Alexafluorochromes (-350, -488 or -568/594) were purchased from Thermo Fisher Scientific (A-11001, A-11004, A-11008 and A-11011) and Jackson ImmunoResearch (115-065-206 and 016-220-084), and used at previously described dilutions (Hajihosseini et al., 2008 (link)).

Immunofluorescence was performed as previously described^{6 (link),53 (link)}. Briefly, cells were fixed with cold methanol, permeabilized, incubated with primary antibodies, and followed with secondary antibodies. Fluorescent secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA) (1:800, A11004)and Jackson ImmunoResearch (West Grove, PA, USA) (1:600, 111-295-003; 1:600, 111-545-003; 1:600, 115-545-003).