UPLC analysis of phenolic compounds was performed using Shimadzu LC-30AC pumps (Shimadzu Co., Kyoto, Japan), and chromatographic separations were performed on a Shim-pack XR-ODS III column (2.0 mm × 75 mm, 1.6 μm). The mobile phase consisted of acetonitrile (solvent A) and 1% phosphatic acid in water (solvent B) at a flow rate of 0.2 mL·min−1. Gradient elution was performed as follows: a linear gradient elution was applied from 2% to 5% solvent A starting from 0 to 3 min; from 5% to 17% solvent A from 3 to 5 min; from 17% to 17.5% solvent A from 5 to 10 min; from 17.5% to 30% solvent A from 10 to 11 min; and from 30% to 60% solvent A from 11 to 15 min. Operating conditions were as follows: column temperature, 30°C; injection volume, 10 μL; and UV-diode array detection at 250 nm. Six biologically active compounds (gallic acid, 5-HMF, morroniside, loganin, sweroside, and cornuside) in the samples were identified by comparing their relative retention times and UV spectra with those of standard compounds and were detected using an external standard method.
Xr ods 3 column
Manufactured by Shimadzu
Sourced in Japan
The XR-ODS III column is a reverse-phase high-performance liquid chromatography (HPLC) column designed for analytical separations. It features a silica-based stationary phase with octadecylsilane (ODS) bonding, providing efficient separation of a wide range of analytes. The column is suitable for use in various HPLC applications.
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2 protocols using xr ods 3 column
1
UPLC Analysis of Phenolic Compounds
2
Quantifying Venlafaxine and Metabolites in Plasma
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We added 10 μL of venlafaxine (400 ng/mL) to 100 μL of plasma and then mixed this with 300 μL methanol used to precipitate proteins. The mixture was vortexed for 5 min and centrifuged at 14 000 rpm for 10 min at 4°C. Then 200 μL of supernatant was transferred into the autosampler vial for analysis. Plasma BUP and HBUP concentrations were determined [20 ,21 (link)] with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) [22 (link),23 (link)] (LC-30A™, Shimadzu, Kyoto, Japan; API 4000™, Applied Biosystems, Framingham, MA, USA), equipped with a Shimpack XR-ODSIII column (1.6 μm, 50×2.0 mm, Japan) and programmed mobile phase conditions of (acetonitrile: 10 mM ammonium formate/B: A): at 0 min, 5% B; at 2.5 min, 30% B; at 3.0 min, 30% B; at 3.5 min, 5% B; at 4.0 min, Stop (v/v) at a flow rate of 0.3 mL/min. Venlafaxine was used as internal standard (IS). The subsequent modes of MRM ion transitions were m/z 240.1–184.2 for BUP, m/z 256.1–238.3 for HBUP, and m/z 278.1–260.5 for IS. The [M+H]+ ions were represented by these transitions.
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