Magnetom lumina

Images were acquired on a 3 Tesla MRI scanner (Magnetom Lumina, Siemens, Erlangen, Germany) using a solenoid (Tx/Rx) coil. The VSOP-T dispersion was diluted in mannitol (60 g/L) and intravenously injected through the tail vein in the anesthetized ApoE KO mouse at a dose of 100 µmol Fe/kg. The imaging was performed in coronal orientation with a 3D fast low-angle shot (FLASH) sequence before, right after, and in time intervals of 10 min post-VSOP administration. Further imaging parameters were repetition time (TR) = 8.4 ms, echo time (TE) = 3.83 ms, flip angle (FA) = 25°, field of view (FOV) = 118 × 58 mm, matrix size = 256 × 128 (reconstructed pixel size = 0.5 × 0.5 mm), and slice thickness = 0.4 mm, 96 slices, bandwidth of 454 Hz/Px, averages = 4, resulting in a scan time of 4:16 min, for the total experiment time of 55 min. Horos Open-Source Medical Image Viewer (version 3.3.6) was utilized for 3D maximum intensity visualizations. The signal-to-background noise ratio (SNR) was calculated by averaging values from three ROIs of equal size defined over the inferior vena cava at each time point. The post-contrast enhancement SNR was corrected by subtraction of pre-contrast enhancement SNR. The half-life was calculated as first-order exponential decay kinetics using GraphPad Prism software version 7 (GraphPad Software, San Diego, CA, USA).

MR imaging was performed using a 3.0 Tesla MR scanner (MAGNETOM Lumina, Siemens, Erlangen, Germany) and a 4-channel receive-coil array for mouse body applications (mouse scapula array, P-H04LE-030, Version1, Rapid Biomedical GmbH, Germany). Following s.c. anesthesia as described above, mice were positioned on the MRI patient table in a prone position. A venous access through the tail vein was established for administration of the contrast agent during the MR imaging. The body temperature (37 °C) was monitored with the use of an MR-compatible heating system (Model 1025, SA Instruments Inc, Stony Brook, NY, USA) to avoid rapid cooling.

Three mice were used for the competition experiment (n = 3). After a tumor size of 1000 mm³ was reached, the animals were anesthetized and examined in an MRI (MAGNETOM Lumina, Siemens, Erlangen, Germany). On day one, imaging without a contrast agent was followed by an intravenous injection of the elastin-specific contrast agent (0.2 mmol/kg). Additional MRI images were acquired as described above (Elastin imaging using T1 weighted sequences) and the animals were then antagonized. On day two, following a native MRI scan, a 5-fold higher dose of an elastin-specific europium-coupled contrast agent was administered through the tail vein. After this imaging, the Gd-containing elastin-specific contrast agent was administered and imaged in an MRI. The data obtained were compared for signal changes.

In vivo MR imaging was performed using a 3.0 Tesla MR scanner (MAGNETOM Lumina, Siemens, Erlangen, Germany) and a 4-channel receive coil array for mouse body applications (Mouse scapula Array, P-H04LE-030, version1, Rapid Biomedical GmbH, Germany). After induction of i.p. anesthesia, the mice were placed in a prone position on the MRI patient table. A venous port was placed via the tail vein for the administration of the contrast agent during MR imaging. The temperature of the body (37 °C) was monitored with an MR-compatible heating system (model 1025, SA Instruments Inc, Stony Brook, NY, USA) to prevent rapid cooling.

All patients were examined using a 3T MR scanner (MAGNETOM Lumina, Siemens Healthineers, Erlangen, Germany). A 64-channel head and neck coil and a 16-channel body coil are used, both of which are placed partially overlapping. The participants were placed in the supine position with the head and neck raised appropriately and their arms placed on both sides. The patients avoided deep breathing and swallowing throughout the process to minimize motion artifacts. Conventional scanning sequences included coronal turbo spin echo (TSE) T1WI; transverse, coronal, and sagittal TSE T2WI; coronal 3D SPACE-STIR T2WI; and T1WI-enhanced sequences. The contrast agent used was domestic gadolinium pentanoate meglumine (Gadolinium-DTPA) at a dose of 0.2 mmol/kg and was rapidly injected intravenously through a high-pressure syringe. Coronary 3D SPACE-STIR T2WI starts scanning three minutes after injecting the contrast agent. Coronal scanning covers the anterior and posterior edges of the spinal canal, the upper edge of the second cervical vertebral body, the upper edge of the second thoracic vertebral body, and the bilateral humeral heads. Axial scanning refers to the coronal position, covering the fifth cervical nerve and the first thoracic nerve root distribution on both sides. The detailed acquisition parameters for the MRI sequences are listed in Table 1.

A video electroencephalogram (VEEG) was undertaken using EEG-1200C (Nippon Optoelectronics Corporation), EEG-V32 (Natus, Nicolet Corporation), EMU40EX (Natus, Nicolet Corporation), and Micromed-SD (Brain Quick, Micromed). Magnetic resonance imaging (MRI), with or without gadolinium injection, was undertaken using an NT 3.0-T Philips Gyroscan (Eindhoven, the Netherlands), GE-SIGNA (GE Healthcare), Vantage Tian 3.0 T (Toshiba, Japan), and Magnetom Lumina (Siemens Healthineers). In addition, 18 F-FDG positron emission tomography (PET) images were acquired using a PET/CT scanner (Elite Discovery, GE HealthCare, Fairfield, Connecticut, USA).