Qubit dsdna hs assay kit fluorometer

DNA was extracted from peripheral blood using the QIAmp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. DNA concentration was quantified with a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) using Qubit dsDNA HS Assay Kit Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) After quantification, a total of 500 ng of genomic DNA was bisulfite-treated using a Zymo EZ-96 DNA Methylation™ Kit (Zymo Research Corp, Irvine, CA, USA) and was purified using a DNA-Clean-Up Kit (Zymo Research Corp, Irvine, CA, USA).
Over 850,000 methylation sites were interrogated with the Infinium Methylation EPIC Bead Chip Kit (Illumina, San Diego, CA, USA) following the Infinium HD Assay Methylation protocol, and raw data were obtained from iS (Illumina) software.

DNA was extracted from peripheral blood using the QIAmp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. DNA concentration was quantified with a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) using Qubit dsDNA HS Assay Kit Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) After quantification, a total of 500 ng of genomic DNA was bisulfite-treated using a Zymo EZ-96 DNA Methylation™ Kit (Zymo Research Corp, Irvine, CA, USA) and was purified using a DNA-Clean-Up Kit (Zymo Research Corp, Irvine, CA, USA).
Over 850,000 methylation sites were interrogated with the Infinium Methylation EPIC Bead Chip Kit (Illumina, San Diego, CA, USA) following the Infinium HD Assay Methylation protocol, and raw data were obtained from iS (Illumina) software (v2.0).