The following materials were used: perilla oil, linseed oil (Shengji Industrial Co., Ltd., Jilin City, Jilin Province); Cremophor RH40 (Dalian Meilun Biotechnology Co., Ltd); Span80 (Tianjin Guangfu Fine Chemical Research Institute); H&E staining kit (Boster Inc, Beijing, China); and neutral-buffered formalin (Beijing Chemical Industry, Beijing, China). Fasting plasma total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) levels were determined using kits from Biosino (Beijing, China). An Elisa kit was obtained from Thermo Fisher Scientific Inc (US).
Ten-week-old Kunming male mice (n = 30) were purchased from the Laboratory Animal Center of Jilin University (Jilin, China). The mice were provided standard rodent chow and water and housed in cages (5 mice per cage) that were placed in an SPF animal facility with the laboratory maintained at a temperature of 23 °C and 40% humidity with a 12 : 12 h light : dark cycle. All animal welfare and experimental procedures were performed strictly according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH publications no. 8023, revised 1978). Moreover, the procedures were approved by the Institutional Animal Care and Use Committee of Jilin University under approved protocol number SY201901004.
Ten-week-old Kunming male mice (n = 30) were purchased from the Laboratory Animal Center of Jilin University (Jilin, China). The mice were provided standard rodent chow and water and housed in cages (5 mice per cage) that were placed in an SPF animal facility with the laboratory maintained at a temperature of 23 °C and 40% humidity with a 12 : 12 h light : dark cycle. All animal welfare and experimental procedures were performed strictly according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH publications no. 8023, revised 1978). Moreover, the procedures were approved by the Institutional Animal Care and Use Committee of Jilin University under approved protocol number SY201901004.